SARS-CoV-2 Spike S1 Protein (B.1.640) (His tag)

Catalog No. ABIN7274872

Product Details

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Protein Characteristics: B.1.640

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His tag.

Purpose: SARS-COV-2 Spike S1 (B.1.640.2/IHU) Protein

Sequence: Gln14-Arg683 (E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G)

Characteristics: Recombinant SARS-COV-2 Spike S1(B.1.640.2/IHU) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Products

Synonyms: E2 Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 74.88 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.