PVRIG Protein (AA 41-171) (Fc Tag)

Catalog No. ABIN7275504

Product Details

Target: PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))

Protein Type: Recombinant

Protein Characteristics: AA 41-171

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This PVRIG protein is labelled with Fc Tag.

Purpose: Cynomolgus PVRIG Protein

Sequence: Thr41-Asp171

Characteristics: Recombinant Cynomolgus PVRIG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr41-Asp171.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus PVRIG, hFc Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. The affinity constant of 0.23 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))

Alternative Name: PVRIG (PVRIG Products)

Synonyms: C7orf15 Protein, PVR related immunoglobulin domain containing Protein, PVRIG Protein

Background: PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

Molecular Weight: 40.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.