Thymic Stromal Lymphopoietin Protein (TSLP) (Arg127Ala-Mutant, Arg130Ala-Mutant) (His-Avi Tag,Biotin)

Catalog No. ABIN7275808

Product Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein Type: Recombinant

Protein Characteristics: Arg127Ala-Mutant, Arg130Ala-Mutant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Thymic Stromal Lymphopoietin protein is labelled with His-Avi Tag,Biotin.

Sequence: Tyr29-Gln159(R127A,R130A)

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP (TSLP Products)

Synonyms: TSLP Protein, thymic stromal lymphopoietin Protein, TSLP Protein, Tslp Protein

Background: Thymic stromal lymphopoietin, TSLP,Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Molecular Weight: 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Tris-Bis PAGE result.