FGF2 Protein (AA 135-288)
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- Target See all FGF2 Proteins
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 135-288
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Human FGF basic (154aa) Protein
- Sequence
- Ala135-Ser288
- Characteristics
- Recombinant Human FGF basic (154aa) Protein is expressed from E.coli without tag.It contains Ala135-Ser288.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FGF basic (154aa) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product FGF2 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- Alternative Name
- FGF basic (FGF2 Products)
- Synonyms
- BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein
- Background
- FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60 % amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
- Molecular Weight
- 17.12 kDa same as Tris-Bis PAGE result.
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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