PRKAA2 Protein (AA 16-195) (His tag)

Catalog No. ABIN7505470

Product Details

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Protein Type: Recombinant

Protein Characteristics: AA 16-195

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PRKAA2 protein is labelled with His tag.

Sequence: Tyr16-Val195

Characteristics: A DNA sequence encoding the Rat AMPK alpha2 (Q09137-1) (Tyr16-Val195) was expressed with a polyhistidine tag at the N-terminus.

Purity: >85 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % tween-80.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: AMPK alpha2 (PRKAA2 Products)

Synonyms: AMPK Protein, AMPK2 Protein, AMPKa2 Protein, PRKAA Protein, Ampk Protein, Ampka2 Protein, 2310008I11Rik Protein, A830082D05 Protein, AMPKalpha2 Protein, AMPKA2 Protein, ampk Protein, ampk2 Protein, prkaa Protein, prkaa1 Protein, PRKAA2 Protein, protein kinase AMP-activated catalytic subunit alpha 2 Protein, protein kinase, AMP-activated, alpha 2 catalytic subunit Protein, protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog Protein, PRKAA2 Protein, Prkaa2 Protein, prkaa2.S Protein, prkaa2 Protein

Background:

Background: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Synonym: 5'-AMP-activated protein kinase catalytic subunit alpha-2,AAPK2,ACACA kinase,Acetyl-CoA carboxylase kinase,AMPK alpha 2 chain,AMPK subunit alpha-2,AMPK2,AMPKa2,AMPKalpha2,HMGCR kinase,Hydroxymethylglutaryl-CoA reductase kinase,PRKAA,PRKAA2,Protein kinase AMP activated alpha 2 catalytic subunit,Protein kinase AMP activated catalytic subunit alpha 2

Molecular Weight: 20 kDa

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect