CRISPR-Cas9 (Active) protein (NLS)
Origin: Streptococcus pyogenes
Host: Escherichia coli (E. coli)
Recombinant
GEEN, EP
Active
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- Target
- CRISPR-Cas9
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Streptococcus pyogenes
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- NLS
- Application
- Genome Editing with Engineered Nucleases (GEEN), Electrophoresis (EP)
- Purpose
- Cas9-C-NLS nuclease
- Characteristics
-
Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli with a C-terminal nucleic localization signal (NLS).
- Purification
- E. coli expression system
- Endotoxin Level
- < 0.1 eu/μg
- Biological Activity Comment
- 20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.
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- Application Notes
-
CRISPR Genome editing
The optimal working dilution should be determined by the end user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 % Glycerol PH 7.4
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- CRISPR-Cas9
- Alternative Name
- Cas9 nuclease
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