CRISPR-Cas9 protein (NLS,His tag,EGFP)
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- Target
- CRISPR-Cas9
- Protein Type
- Recombinant
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Origin
- Streptococcus pyogenes
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- NLS,His tag,EGFP
- Application
- Genome Editing with Engineered Nucleases (GEEN)
- Purpose
- NLS-Cas9-EGFP Nuclease
- Characteristics
- Fusion protein Cas9 nuclease expressed in an Escherichia coli with a N terminal end nuclear localization signal (NLS) and a C terminal end an EGFP and a 6x(His) sequence.
- Purification
- Escherichia coli expression system
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- Application Notes
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CRISPR Genomic editing
The optimal working dilution should be determined by the end user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 10 mM Tris, 300 mM NaCl, 1 mM DTT, pH 7.4 (50 % glycerol).
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- CRISPR-Cas9
- Alternative Name
- Cas9 nuclease
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