CRISPR-Cas9 protein (NLS)
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- Target
- CRISPR-Cas9
- Protein Type
- Recombinant
- Origin
- Streptococcus aureus
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- NLS
- Application
- Genome Editing with Engineered Nucleases (GEEN)
- Purpose
- SaCas9 2NLS Nuclease
- Characteristics
- Recombinant Streptococcus aureus tag-free Cas9 nuclease expressed in an Escherichia coli with both N-terminal and C-terminal nucleic localization signal (NLS).
- Purification
- Escherichia coli expression system
- Endotoxin Level
- 10 EU/mg as analyzed by gel clotting method
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- Application Notes
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CRISPR Genomic editing
The optimal working dilution should be determined by the end user. - Assay Procedure
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Cleavage assay: A 20 uL reaction in 1xSaCas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and SaCas9 2NLS Nuclease for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 20 mM Tris, 300 mM NaCl, 0.1 mM TCEP, pH 7.5 (50 % glycerol).
- Storage
- -20 °C
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- Target
- CRISPR-Cas9
- Alternative Name
- Cas9 nuclease
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