CRISPR-Cas9 protein (NLS)
Origin: Streptococcus pyogenes
Host: Escherichia coli (E. coli)
Recombinant
GEEN
-
- Target
- CRISPR-Cas9
- Protein Type
- Recombinant
-
Origin
- Streptococcus pyogenes
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- NLS
- Application
- Genome Editing with Engineered Nucleases (GEEN)
- Purpose
- Ultra NLS-Cas9-basic
- Characteristics
- Recombinant Streptococcus pyogenes Cas9 gene expressed in an Escherichia coli with a N-terminal nucleic localization signal (NLS).
- Purification
- Escherichia coli expression system
- Endotoxin Level
- 10 EU/mg as analyzed by gel clotting method
-
-
- Application Notes
-
CRISPR Genomic editing
The optimal working dilution should be determined by the end user. - Assay Procedure
-
Cleavage assay: A 20 uL reaction in 1xCas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng Ultra NLS-Cas9-basic for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- In 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH 8.0 (50 % glycerol).
- Preservative
- Other preservative
- Storage
- -20 °C
-
- Target
- CRISPR-Cas9
- Alternative Name
- Cas9 nuclease
-
You are here:
