GPC1 Protein (His tag)

Catalog No. ABIN7534510

Product Details

Target: GPC1 (Glypican 1 (GPC1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GPC1 protein is labelled with His tag.

Purpose: Active Recombinant Human Glypican-1/GPC1 Protein

Sequence: DPASKSRSCG EVRQIYGAKG FSLSDVPQAE ISGEHLRICP QGYTCCTSEM EENLANRSHA ELETALRDSS RVLQAMLATQ LRSFDDHFQH LLNDSERTLQ ATFPGAFGEL YTQNARAFRD LYSELRLYYR GANLHLEETL AEFWARLLER LFKQLHPQLL LPDDYLDCLG KQAEALRPFG EAPRELRLRA TRAFVAARSF VQGLGVASDV VRKVAQVPLG PECSRAVMKL VYCAHCLGVP GARPCPDYCR NVLKGCLANQ ADLDAEWRNL LDSMVLITDK FWGTSGVESV IGSVHTWLAE AINALQDNRD TLTAKVIQGC GNPKVNPQGP GPEEKRRRGK LAPRERPPSG TLEKLVSEAK AQLRDVQDFW ISLPGTLCSE KMALSTASDD RCWNGMARGR YLPEVMGDGL ANQINNPEVE VDITKPDMTI RQQIMQLKIM TNRLRSAYNG NDVDFQDASD DGSGSGSGDG CLDDLCGRKV SRKSSSSRTP LTHALPGLSE QEGQ

Specificity: Asp24-Gln527

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 1 μg/mL (100 μL/well) can bind Human Glypican 1 with a linear range of 2-80 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: GPC1 (Glypican 1 (GPC1))

Alternative Name: Glypican-1/GPC1 (GPC1 Products)

Synonyms: gpc1 Protein, zgc:63947 Protein, GPC1 Protein, Glypican-1 Protein, glypican Protein, AI462976 Protein, HSPG M12 Protein, HSPGM12 Protein, glypican 1b Protein, glypican 1 Protein, gpc1b Protein, GPC1 Protein, gpc1 Protein, Gpc1 Protein

Background:

Description: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Name: GPC1,glypican

Gene ID: 2817

UniProt: P35052

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation