IL5RA Protein (His tag)

Catalog No. ABIN7520219

Product Details

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This IL5RA protein is labelled with His tag.

Purpose: Recombinant Mouse IL-5RA/CD125 Protein

Sequence: DLLNHKKFLL LPPVNFTIKA TGLAQVLLHW DPNPDQEQRH VDLEYHVKIN APQEDEYDTR KTESKCVTPL HEGFAASVRT ILKSSHTTLA SSWVSAELKA PPGSPGTSVT NLTCTTHTVV SSHTHLRPYQ VSLRCTWLVG KDAPEDTQYF LYYRFGVLTE KCQEYSRDAL NRNTACWFPR TFINSKGFEQ LAVHINGSSK RAAIKPFDQL FSPLAIDQVN PPRNVTVEIE SNSLYIQWEK PLSAFPDHCF NYELKIYNTK NGHIQKEKLI ANKFISKIDD VSTYSIQVRA AVSSPCRMPG RWGEWSQPIY VGKERKSLVE WH

Specificity: Asp18-His339

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Alternative Name: IL-5RA/CD125 (IL5RA Products)

Synonyms: MGC86517 Protein, IL5RA Protein, il5r Protein, cdw125 Protein, hsil5r3 Protein, gpIL-5ra Protein, CD125 Protein, CDw125 Protein, HSIL5R3 Protein, IL5R Protein, Il5r Protein, interleukin 5 receptor subunit alpha L homeolog Protein, interleukin 5 receptor subunit alpha Protein, interleukin 5 receptor, alpha Protein, il5ra.L Protein, IL5RA Protein, il5ra Protein, Il5ra Protein

Background:

Description: Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand-specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony-stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine that is particularly important in the development of asthma for the terminal differentiation, activation, and survival of committed eosinophil precursors.

Name: Il5r,CD125,CDw125,IL5RA

Gene ID: 16192

UniProt: P21183

Pathways: JAK-STAT Signaling