TRKA Protein (Fc Tag,His tag)

Catalog No. ABIN7533912

Product Details

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TRKA protein is labelled with Fc Tag,His tag.

Purpose: Active Recombinant Human Trk-A/NTRK1 Protein

Sequence: AAPCPDACCP HGSSGLRCTR DGALDSLHHL PGAENLTELY IENQQHLQHL ELRDLRGLGE LRNLTIVKSG LRFVAPDAFH FTPRLSRLNL SFNALESLSW KTVQGLSLQE LVLSGNPLHC SCALRWLQRW EEEGLGGVPE QKLQCHGQGP LAHMPNASCG VPTLKVQVPN ASVDVGDDVL LRCQVEGRGL EQAGWILTEL EQSATVMKSG GLPSLGLTLA NVTSDLNRKN VTCWAENDVG RAEVSVQVNV SFPASVQLHT AVEMHHWCIP FSVDGQPAPS LRWLFNGSVL NETSFIFTEF LEPAANETVR HGCLRLNQPT HVNNGNYTLL AANPFGQASA SIMAAFMDNP FEFNPEDPIP DTNSTSGDPV EKKDE

Specificity: Ala33-Glu407

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 12-48 ng/mL in the presence of 10 ng/mL of recombinant human NGF.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Alternative Name: Trk-A/NTRK1 (NTRK1 Products)

Synonyms: MTC Protein, TRK Protein, TRK1 Protein, TRKA Protein, Trk-A Protein, p140-TrkA Protein, CDHF12 Protein, CDHR16 Protein, HSCR1 Protein, MEN2A Protein, MEN2B Protein, MTC1 Protein, PTC Protein, RET-ELE1 Protein, RET51 Protein, Trk Protein, C80751 Protein, Tkr Protein, TrkA Protein, trk Protein, trka Protein, CTRKA Protein, trkA Protein, neurotrophic receptor tyrosine kinase 1 Protein, ret proto-oncogene Protein, neurotrophic tyrosine kinase, receptor, type 1 Protein, NTRK1 Protein, RET Protein, Ntrk1 Protein, ntrk1 Protein

Background:

Description: This protein is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

Name: MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA,NTRK1,TRK,TRK1,TRKA,Trk-A,p140-TrkA

Gene ID: 4914

UniProt: P04629

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process