HGF Protein

Catalog No. ABIN935430

Product Details

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Hi-5 Cells

Characteristics: Purified recombinant Human HGF protein
Expression System: Hi-5 cells
Bioactivity: The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/mL.

Purity: > 98 % pure

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.5 mg/mL.

Buffer: Lyophilized from 0.1 M L-Arginine HCL, with 10 mM tris, pH8.0 and 200 mM NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: HGF (HGF Products)

Synonyms: DFNB39 Protein, F-TCF Protein, HGFB Protein, HPTA Protein, SF Protein, HGF Protein, C230052L06Rik Protein, HGF/SF Protein, NK1 Protein, NK2 Protein, SF/HGF Protein, hepatocyte growth factor Protein, HGF Protein, Hgf Protein

Background: HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and Mouse HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (a-chain and beta-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer.
Alternative Names: SF protein, Scatter Factor protein, Hepatopoietin protein, Hepatocyte Growth Factor protein, HPTA protein

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor