EGF Protein (AA 971-1023)

Catalog No. ABIN987814

Product Details

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 971-1023

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 <0.6 ng/mL, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of >1.7 x 10^6 units/mg.
AA 971-1023, expressed with an N-terminal Met

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.1 ng/μg (1 EU/μg) determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80 °C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4 °C or up to 6 months at -20 °C.

Expiry Date: 12 months

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Epidermal Growth Factor (EGF Products)

Synonyms: HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein

Background: Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-a and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds. Recombinant human Epidermal Growth Factor (rhEGF) produced in E. coli is a non-glycosylated polypeptide chain of 54 amino acids. A fully biologically active molecule, rhEGF has a molecular mass of 6.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Urogastrone, URG

Molecular Weight: 6.2kDa, observed by reducing SDS-PAGE.

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation