IL-10 Protein

Catalog No. ABIN988020

Product Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Characteristics: The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be

Purity: > 98.0 % as determined by:(a) Analysis by RP-HPLC.(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Endotoxin Level: Level Less than 0.1 ng/ug (1 IEU/ug) of mouse Interleukin-10 (IL-10)

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended to reconstitute the lyophilized Mouse Interleukin-10 (IL-10) in sterile 18 M-omega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Storage: -20 °C

References

Larson, Hübner, Torrero, Morris, Brankin, Swierczewski, Davies, Vonakis, Mitre: "Chronic helminth infection reduces basophil responsiveness in an IL-10-dependent manner." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4188-99, (2012) (PubMed).

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: Interleukin-10 (IL-10) (IL10 Products)

Synonyms: CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein

Background: Interleukin-10 (IL-10) is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 (IL-10) in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce Interleukin-10 (IL-10). In human Interleukin-10 (IL-10) is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by mococytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cells lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secerete large quantities of Interleukin-10 (IL-10) into the conditioned medium. The synthesis of Interleukin-10 (IL-10) by monocytes is inhibited by IL-4 and IL-10. Mouse Interleukin-10 (IL-10) produced in E. coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18,885 Da. Synonym: rmIL-10, IL-1. Formulation: The protein was lyophilized after extensive dialysis against 20mM PB, pH7.3, 300mM NaCl buffer.

Molecular Weight: 18,885 Da.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints