anti-Mouse (Murine) Methionyl Aminopeptidase 2 antibody for Western Blotting

Recommended Methionyl Aminopeptidase 2 Antibody (supplied by: Log in to see )

Antigen
Methionyl Aminopeptidase 2 (METAP2) Antibodies
  • metap2
  • MGC53792
  • METAP2
  • GB13458
  • DDBDRAFT_0190923
  • DDBDRAFT_0304991
  • DDB_0190923
  • DDB_0304991
  • 4930584B20Rik
  • A930035J23Rik
  • AI047573
  • AL024412
  • AU014659
  • Amp2
  • Mnpep
  • p67
  • p67eIF2
  • MAP2
  • MNPEP
  • wu:fb98h06
  • zgc:66250
  • methionyl aminopeptidase 2 L homeolog
  • methionyl aminopeptidase 2
  • methionine aminopeptidase 2
  • methionine aminopeptidase
  • methionyl aminopeptidase 2 S homeolog
  • methionyl aminopeptidase 2b
  • metap2.L
  • METAP2
  • metap2
  • LOC551771
  • CAALFM_C604080WA
  • Metap2
  • metap2.S
  • metap2b
Epitope
N-Term
22
10
10
10
8
7
7
6
5
4
2
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
88
16
8
1
1
1
1
1
1
1
1
1
Host
Rabbit
82
9
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
6
6
6
Application
Immunohistochemistry (IHC), Western Blotting (WB)
84
57
28
11
8
6
4
2
2
Options
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Catalog No. ABIN2784850
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.920566 ABIN2001079 ELISA IHC (p) IP WB Rabbit IgG AA 2-478 Log in to see Polyclonal 0
2.920566 ABIN631470 WB Rabbit N-Term Log in to see Polyclonal 0
2.920566 ABIN2463504 ELISA WB Rabbit Log in to see Polyclonal 0
2.920566 ABIN2692624 IP WB Rabbit IgG AA 2-478 Log in to see 010 0
2.920566 ABIN4904354 WB Rabbit IgG Log in to see Polyclonal 0
2.920566 ABIN5996053 WB Rabbit IgG Log in to see Polyclonal 0
2.920566 ABIN5965103 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN958392 IHC IHC (p) WB Rabbit IgG AA 95-144 Log in to see Polyclonal 0
1 ABIN5661334 WB Rabbit Log in to see Polyclonal 0
1 ABIN5700075 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2914681 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1088565 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5892289 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6033184 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Methionyl Aminopeptidase 2 (METAP2) Antibodies
Epitope N-Term
(22), (10), (10), (10), (8), (7), (7), (6), (5), (4), (2), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(88), (16), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(82), (9), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(7), (7), (7), (6), (6), (6)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(84), (57), (28), (11), (8), (6), (4), (2), (2)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN979997
Sequence ATGKKKKKKK KKRGPKVQTD PPSVPICDLY PNGVFPKGQE CEYPPTQDGR
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against METAP2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human METAP2

Target Details

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Antigen
Alternative Name METAP2 (METAP2 Antibody Abstract)
Background METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site.This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MAP2, MNPEP, p67, p67eIF2
Protein Interaction Partner: UBC, DAK, NPEPPS, GBP1, GAPDH, HMGA1, MTOR, EEF2K, APP, CALM1, S100A4, RAC1, MAPK3, EIF2S3, MAPK1, EIF2AK2, CDC42, E2F4,
Protein Size: 478
Molecular Weight 53 kDa
Gene ID 10988
NCBI Accession NM_006838, NP_006829
UniProt P50579
Pathways Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 478 AA

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Kass, Bridges, Borczuk, Greenberg: "Methionine aminopeptidase-2 as a selective target of myofibroblasts in pulmonary fibrosis." in: American journal of respiratory cell and molecular biology, Vol. 37, Issue 2, pp. 193-201, 2007

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Methionyl Aminopeptidase 2 (METAP2) (N-Term) antibody (ABIN2784850) WB Suggested Anti-METAP2 Antibody Titration: 0.2-1 ug/ml
Positive Contro...
Immunohistochemistry (IHC) image for anti-Methionyl Aminopeptidase 2 (METAP2) (N-Term) antibody (ABIN2784850) Immunohistochemistry with Small intestine tissue at an antibody concentration of 5µg/...