anti-Rat (Rattus) CACNA1A antibody for Immunohistochemistry

Recommended CACNA1A Antibody (supplied by: Log in to see )

Antigen
Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) Antibodies
  • cav2.1
  • cacna-a
  • ca(v)2.1
  • cacna1a
  • si:ch211-237k17.1
  • BccA1
  • Cav2.1
  • rbA-1
  • APCA
  • BI
  • CACNL1A4
  • CAV2.1
  • EA2
  • FHM
  • HPCA
  • MHP
  • MHP1
  • SCA6
  • Caca1a
  • Cacnl1a4
  • Ccha1a
  • alpha1A
  • la
  • leaner
  • nmf352
  • rkr
  • rocker
  • tg
  • tottering
  • CACH4
  • CACN3
  • calcium channel, voltage-dependent, P/Q type, alpha 1A subunit S homeolog
  • calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a
  • calcium voltage-gated channel subunit alpha1 A
  • calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
  • P/Q-type voltage-gated calcium channel alpha1A subunit ChCaChA1A
  • cacna1a.S
  • cacna1aa
  • CACNA1A
  • Cacna1a
  • LOC395892
Epitope
AA 865-881, Intracellular
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
27
22
20
1
1
1
1
1
1
Host
Rabbit
28
1
Clonality
Polyclonal
Conjugate
This CACNA1A antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
13
11
7
4
2
1
1
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Catalog No. ABIN1742170
$ 821.43
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.926102 ABIN2434370 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN2964787 IHC WB Rabbit IgG AA 717-1190 Log in to see Polyclonal 0
1 ABIN2219774 IP IHC WB Rabbit IgG AA 865-881, Intracellular Log in to see Polyclonal 0
1 ABIN1841965 IHC WB Rabbit AA 2150-2210 Log in to see Polyclonal 0
1 ABIN1841964 IHC WB Rabbit AA 1300-1400 Log in to see Polyclonal 0

General

Antigen Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) Antibodies
Epitope AA 865-881, Intracellular
(2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(27), (22), (20), (1), (1), (1), (1), (1), (1)
Host Rabbit
(28), (1)
Clonality
Polyclonal
Conjugate This CACNA1A antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(13), (11), (7), (4), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-CACNA1A Antibody

Target Details CACNA1A Application Details Handling References for anti-CACNA1A antibody (ABIN1742170) Images
Sequence (C)PSSPERAPGR EGPYGRE
Cross-Reactivity (Details) Mouse - AA 16-17 residues identical, human - AA 16-17 residues identical.
Characteristics Peptide Confirmation:
Confirmed by mass-spectrography and amino acid analysis.

Antibody concentration after reconstitution:
0.8 mg/mL.

Preadsorption Control:
1 µg peptide per 10 µg antibody.

GPCR
Purification Affinity purified on immobilized antigen.
Immunogen Peptide corresponding to amino acid residues 865-881 of rat CaV2.1. Intracellular loop between domains II and III.

Target Details CACNA1A

Product Details anti-CACNA1A Antibody Application Details Handling References for anti-CACNA1A antibody (ABIN1742170) Images back to top
Antigen
Alternative Name CaV2.1 (CACNA1A Antibody Abstract)
Background Synonyms:
Alpha1A, Cacna1a

Voltage dependent Ca2+ channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation. The voltage dependent calcium channels are composed of several subunits, alpha1, beta, alpha2δ and gamma. Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type. The Cav2.1 (formally named alpha1A) makes up the alpha1 poreforming subunit in P/Q type Ca2+ channel family. It is expressed preferentially in the central nervous system where along with Cav2.2 is responsible for pre-synaptic Ca2+ influx and neurotransmitter release. Mutations in the Cav2.1 have been shown to cause several neurological disorders among them are familial hemiplegic migraine, episodic ataxia type 2, and spinocerebellar ataxia type 6 (SCA6). The involvement of Cav2.1 in synaptic transmission was assessed by using ω-Agatoxin IVA, a specific blocker of the Cav2.1 channel. The blocking sensitivity is dependent on the a subunit isoform and on the splice variant.
Gene ID 25398
UniProt P54282
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process

Application Details

Product Details anti-CACNA1A Antibody Target Details CACNA1A Handling References for anti-CACNA1A antibody (ABIN1742170) Images back to top
Application Notes Recommended Dilution:
WB: approx. 1:200,
IHC: approx. 1:100
Restrictions For Research Use only

Handling

Product Details anti-CACNA1A Antibody Target Details CACNA1A Application Details References for anti-CACNA1A antibody (ABIN1742170) Images back to top
Format Lyophilized
Reconstitution 0.2 mL deionized water.
Handling Advice Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
Storage 4 °C/-20 °C
Storage Comment The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, it is recommended to add glycerol at a ratio of 1:1 and to keep the antibody solution at -20 °C.

References for anti-CACNA1A antibody (ABIN1742170)

Product Details anti-CACNA1A Antibody Target Details CACNA1A Application Details Handling Images back to top
Background publications

Scheuber, Miles, Poncer: "Presynaptic Cav2.1 and Cav2.2 differentially influence release dynamics at hippocampal excitatory synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 46, pp. 10402-9, 2004

Tottene, Fellin, Pagnutti, Luvisetto, Striessnig, Fletcher, Pietrobon: "Familial hemiplegic migraine mutations increase Ca(2+) influx through single human CaV2.1 channels and decrease maximal CaV2.1 current density in neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 13284-9, 2002

Bourinet, Soong, Sutton, Slaymaker, Mathews, Monteil, Zamponi, Nargeot, Snutch: "Splicing of alpha 1A subunit gene generates phenotypic variants of P- and Q-type calcium channels." in: Nature neuroscience, Vol. 2, Issue 5, pp. 407-15, 1999

Images

Product Details anti-CACNA1A Antibody Target Details CACNA1A Application Details Handling References for anti-CACNA1A antibody (ABIN1742170) back to top
Supplier Images
Western Blotting (WB) image for anti-Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (AA 865-881), (Intracellular) antibody (ABIN1742170) Western blot analysis of rat brain membranes: 1. Anti-CaV2.1 antibody, (1:200). 2. An...
Immunohistochemistry (IHC) image for anti-Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (AA 865-881), (Intracellular) antibody (ABIN1742170) Expression of Cav2.1 in mouse cerebellum Immunohistochemical staining of mouse cerebe...