We are preparing the requested document. Please wait, this may take a while...!
|Antigen||E1A Binding Protein P300 (EP300) Antibodies|
|Epitope||AA 2065-2414 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This p300 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-p300 AntibodyTarget Details p300 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Histone acetyltransferase p300(EP300) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Histone acetyltransferase p300(EP300) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: E1A binding protein p300
Protein Name: Histone acetyltransferase p300
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human KAT3B recombinant protein (Position: L2065-H2414). Human KAT3B shares 94% amino acid (aa) sequence identity with mouse KAT3B.|
|Plasmids, Primers & others|
Target Details p300Product Details anti-p300 Antibody Application Details Handling Images back to top
|Alternative Name||EP300 (EP300 Antibody Abstract)|
E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
Synonyms: CREBBP/EP300 inhibitory protein 1 antibody|Cyclic AMP responsive enhancer binding protein antibody|E1A associated protein p300 antibody|E1A binding protein p300 antibody|E1A-associated protein p300 antibody|EC 18.104.22.168 antibody|EP300 antibody|EP300: E1A binding protein p300 antibody|EP300_HUMAN antibody|Histone acetyltransferase p300 antibody|KAT3B antibody|p300 HAT antibody|RB and P300 binding protein EID 1 antibody|Retinoblastoma protein associated protein antibody|RSTS2 antibody
|Pathways||p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size|
Application DetailsProduct Details anti-p300 Antibody Target Details p300 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for KAT3B is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-p300 Antibody Target Details p300 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-p300 Antibody Target Details p300 Application Details Handling back to top