anti-Human Sterol Regulatory Element Binding Transcription Factor 2 antibody for ELISA

Recommended Sterol Regulatory Element Binding Transcription Factor 2 Antibody (supplied by: Log in to see )

Antigen
Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) Antibodies
  • SREBF2
  • srebp2
  • SREBP2
  • zgc:158371
  • AI608257
  • SREBP-2
  • SREBP2gc
  • bHLHd2
  • lop13
  • nuc
  • Srebf2_retired
  • sterol regulatory element binding transcription factor 2
  • sterol regulatory element binding factor 2
  • sterol regulatory element binding transcription factor 2 L homeolog
  • SREBF2
  • srebf2
  • Srebf2
  • srebf2.L
Reactivity
Chicken, Human
95
30
29
23
7
6
6
5
5
4
4
2
1
1
1
1
1
Host
Mouse
70
25
1
Clonality
Monoclonal
Conjugate
Un-conjugated
5
5
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
84
54
45
44
32
10
9
2
1
1
1
1
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Available images

Catalog No. ABIN4355936
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2463718 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN5690270 ELISA IF IHC (p) WB Rabbit IgG AA 440-490 Log in to see Polyclonal 0
1 ABIN4355937 ELISA ICC IF IHC WB Rabbit Center Log in to see Polyclonal 0
1 ABIN3032752 IF IHC ELISA WB Rabbit Ig Fraction AA 399-427 Log in to see Polyclonal 0
1 ABIN2628740 ELISA IF IHC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN4355941 ELISA ICC IF WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355942 ELISA ICC IF WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355943 ELISA ICC IF WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355946 ELISA ICC IF WB Biotin Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355954 ELISA ICC IF WB DyLight 350 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355949 ELISA ICC IF WB DyLight 488 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355953 ELISA ICC IF WB DyLight 550 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355948 ELISA ICC IF WB DyLight 680 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN4355950 ELISA ICC IF WB DyLight 755 Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN562995 ELISA WB Mouse AA 801-900, partial Log in to see Polyclonal 0
1 ABIN2366522 IF IHC ELISA WB Rabbit IgG AA 402-431, Center, Internal Region Log in to see Polyclonal 0
1 ABIN4245321 ELISA WB HRP Mouse IgG1 kappa Log in to see 1D2 0
1 ABIN1938130 ELISA ICC IF IHC WB FITC Rabbit IgG AA 399-427 Log in to see Polyclonal 0
1 ABIN1938131 ELISA ICC IF IHC WB PE Rabbit IgG AA 399-427 Log in to see Polyclonal 0
1 ABIN1938127 ELISA ICC IF IHC WB Alkaline Phosphatase (AP) Rabbit IgG AA 399-427 Log in to see Polyclonal 0

General

Antigen Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) Antibodies
Reactivity Chicken, Human
(95), (30), (29), (23), (7), (6), (6), (5), (5), (4), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(70), (25), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(84), (54), (45), (44), (32), (10), (9), (2), (1), (1), (1), (1)
Pubmed 14 references available
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Product details

Target Details Application Details Handling References Images
Specificity Recognizes the precursor and mature forms of human SREBP2.
Purification Protein G purified
Immunogen Human SREBP2 protein (amino acids 48-403). [UniProt# Q12772]
Clone 1D2
Isotype IgG1 kappa

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name SREBP2 (SREBF2 Antibody Abstract)
Background Gene Symbol: SREBF2
Gene ID 6721
UniProt Q12772
Pathways Regulation of Lipid Metabolism by PPARalpha

Application Details

Product details Target Details Handling References Images back to top
Application Notes Western Blot 2-5 μg/mL, ELISA, Immunocytochemistry/Immunofluorescence 10 μg/mL, Immunoprecipitation 10 μg/1 mg protein, Gel Super Shift AssaysIn WB, this antibody is expected to detect the precursor as well as the mature forms of SREBP2. Use of this antibody in EMSA/GS (Gel Supershift Assay) and IP applications has been reported in PMIDs 15333705 and 15213220 respectively.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

Product details Target Details Application Details Handling Images back to top
Product cited in:

Du, Kazim, Dawes, Brown, Yang: "The AAA ATPase VPS4/SKD1 regulates endosomal cholesterol trafficking independently of ESCRT-III." in: Traffic (Copenhagen, Denmark), Vol. 14, Issue 1, pp. 107-19, 2013 (Sample species: Human). Further details: Western Blotting

Luu, Sharpe, Stevenson, Brown: "Akt acutely activates the cholesterogenic transcription factor SREBP-2." in: Biochimica et biophysica acta, Vol. 1823, Issue 2, pp. 458-64, 2012 (Sample species: Human). Further details: Western Blotting

Du, Kazim, Brown, Yang: "An essential role of Hrs/Vps27 in endosomal cholesterol trafficking." in: Cell reports, Vol. 1, Issue 1, pp. 29-35, 2012 (Sample species: Human). Further details: Western Blotting

Song, DeBose-Boyd: "Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase stimulated by delta- and gamma-tocotrienols." in: The Journal of biological chemistry, Vol. 281, Issue 35, pp. 25054-61, 2006 (Sample species: Human). Further details: Western Blotting

Sun, Li, Goldstein, Brown: "Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro." in: The Journal of biological chemistry, Vol. 280, Issue 28, pp. 26483-90, 2005 (Sample species: Human). Further details: Western Blotting

Song, Javitt, DeBose-Boyd: "Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol." in: Cell metabolism, Vol. 1, Issue 3, pp. 179-89, 2005 (Sample species: Human). Further details: Western Blotting

Adams, Reitz, De Brabander, Feramisco, Li, Brown, Goldstein: "Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs." in: The Journal of biological chemistry, Vol. 279, Issue 50, pp. 52772-80, 2004 (Sample species: Human). Further details: Western Blotting

Demoulin, Ericsson, Kallin, Rorsman, Rönnstrand, Heldin: "Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins." in: The Journal of biological chemistry, Vol. 279, Issue 34, pp. 35392-402, 2004 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Zhang, Hillgartner: "Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens." in: The Journal of nutrition, Vol. 134, Issue 9, pp. 2205-10, 2004 (Sample species: Chicken). Further details: Western Blotting

Sever, Yang, Brown, Goldstein, DeBose-Boyd: "Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain." in: Molecular cell, Vol. 11, Issue 1, pp. 25-33, 2003 (Sample species: Human). Further details: Western Blotting

Janowski: "The hypocholesterolemic agent LY295427 up-regulates INSIG-1, identifying the INSIG-1 protein as a mediator of cholesterol homeostasis through SREBP." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 12675-80, 2002 (Sample species: Human). Further details: Western Blotting

Yin, Zhang, Hillgartner: "Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19554-65, 2002 (Sample species: Chicken). Further details: Western Blotting

Yabe, Brown, Goldstein: "Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 12753-8, 2002 (Sample species: Human). Further details: Western Blotting

Janowski, Shan, Russell: "The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 45408-16, 2001 (Sample species: Human). Further details: Western Blotting

Images

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Supplier Images
Immunofluorescence (IF) image for anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) antibody (ABIN4355936) Immunocytochemistry/Immunofluorescence: SREBP2 Antibody (1D2) [NBP1-54446] - SREBP2 (...
Western Blotting (WB) image for anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) antibody (ABIN4355936) Western Blot: SREBP2 Antibody (1D2) [NBP1-54446] - Lane1: HEp-2 cells. Lane 2: MCF-7 ...