anti-Guinea Pig TACR1 antibody for Western Blotting

Recommended TACR1 Antibody (supplied by: Log in to see )

Antigen
Tachykinin Receptor 1 (TACR1) Antibodies
  • NK1R
  • NKIR
  • SPR
  • TAC1R
  • Nk1r
  • Spr
  • Tac1r
  • nk1R
  • Tacr1
  • ASPR
  • tachykinin receptor 1
  • TACR1
  • Tacr1
Epitope
C-Term
22
17
15
14
11
11
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
140
68
59
10
10
6
5
5
4
4
2
2
1
1
1
1
Host
Rabbit
128
15
8
Clonality
Polyclonal
Conjugate
This TACR1 antibody is un-conjugated
8
8
7
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
97
48
45
32
23
19
16
13
5
2
1
1
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Catalog No. ABIN152451
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN297740 ELISA WB Goat AA 227-239 Log in to see Polyclonal 0
1 ABIN2628932 WB Rabbit IgG AA 231-245 Log in to see Polyclonal 0
1 ABIN1737300 FACS IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN1737299 IHC (fro) IF WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Tachykinin Receptor 1 (TACR1) Antibodies
Epitope C-Term
(22), (17), (15), (14), (11), (11), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
(140), (68), (59), (10), (10), (6), (5), (5), (4), (4), (2), (2), (1), (1), (1), (1)
Host Rabbit
(128), (15), (8)
Clonality
Polyclonal
Conjugate This TACR1 antibody is un-conjugated
(8), (8), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(97), (48), (45), (32), (23), (19), (16), (13), (5), (2), (1), (1)
Pubmed 30 references available
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Product Details anti-TACR1 Antibody

Target Details TACR1 Application Details Handling References for anti-TACR1 antibody (ABIN152451) Images
Specificity This antibody is specific to the NK-1 receptor. Staining of cells transfected with NK-1 was abolished by preabsorption of the antisera with NK-1. Specificity was also verified by the localization of immunohistochemical staining with NB300-101 in neurons in
Purification Unpurified
Immunogen A synthetic peptide corresponding to the C-terminus of rat Neurokinin 1 Receptor, conjugated to bovine thyroglobulin. [UniProt# P14600]

Target Details TACR1

Product Details anti-TACR1 Antibody Application Details Handling References for anti-TACR1 antibody (ABIN152451) Images back to top
Antigen
Alternative Name NK1R (TACR1 Antibody Abstract)
Background Gene Symbol: TACR1
Gene ID 6869
UniProt P14600
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration

Application Details

Product Details anti-TACR1 Antibody Target Details TACR1 Handling References for anti-TACR1 antibody (ABIN152451) Images back to top
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:1000This Neurokinin 1 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. For ICC/IF staining, please see customer the review. Use in Immunohistochemistry on paraffin embedded sections was reported in scientific literature (PMID: 23792204).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Neurokinin 1 Receptor Antibody Immunohistochemistry1) Perfuse rat through the ascending aorta with 500 mL. of 0.1 M phosphate buffered saline (PBS) (pH 7.4 at 4 degrees C), followed by 750 mL. of PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9, 4 degrees C). Tissue of interest is then dissected and post-fixed in PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9 at 4 degrees C) for at least 4 hours, followed by fixation in PBS containing 30 % sucrose (pH 7.4, 4 degrees C) for at least 24 hours. Until the tissue sinks.
2) Cut fixed sections at 60 um using a sliding microtome. 3) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.
) -use same protocol without agitation. 4) Place tissue section in microcentrifuge tubes containing 1 mL. Of PBS, pH 7.4 as they are being cut. Wash sections (in PBS) on an upright rotator for 10-15 minutes.
) Remove PBS and add 1 mL. of blocking solution (PBS + 1 % normal donkey serum (NDS) + 0.3 % triton X-100).
) Incubate tissue sections in blocking solution for 30 minutes at room temperature (RT) on rotator. 7) Remove blocking solution and add anti-neurokinin-1 receptor or anti-Neurokinin-3 receptor antibody +PBS + 1 % NDS + 0.3 % triton X-100 + NB 300-101/102 at 1:100~1:10008) Incubate overnight on rotator at RT.
) Remove primary antisera and perform 3 x 10 minute PBS washes (1ml volume) on rotator.
) Add 1 mL. of secondary antibody and incubate for 2 hours at RT on rotator. -PBS + 1 % NDS + 0.3 % triton X 100 + anti-rabbit Cy3 (Jackson Immunoresearch Labs) at 1:60011) Perform 3 X 10 minute PBS washes (1 mL volume) on rotator12) Mount tissue sections on gelatin coated slides and allow tissue to dry13) Run slides through alcohol gradients (70 %, 90 %, 100 %, xylene) leaving slide in each alcohol and xylene for 2 minutes14) Coverslip with DPX mountant (FLUKA) ** Note: 24 well plates may be substituted for microcentrifuge tubes. Use flat top bench rotator instead of upright rotator.
Restrictions For Research Use only

Handling

Product Details anti-TACR1 Antibody Target Details TACR1 Application Details References for anti-TACR1 antibody (ABIN152451) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-TACR1 antibody (ABIN152451)

Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Handling Images back to top
Product cited in:

Morinaga, Nakamuta, Yamamoto: "Hypoxia-induced increases in serotonin-immunoreactive nerve fibers in the medulla oblongata of the rat." in: Acta histochemica, Vol. 118, Issue 8, pp. 806-817, 2016 (Sample species: Human).

Wu, Zhang, Qian, Guo, Yang, Huang, Luo, Xiang, Deng, Dai, Peng, Pan: "Antinociceptive effects of endomorphin-2: suppression of substance P release in the inflammatory pain model rat." in: Neurochemistry international, Vol. 82, pp. 1-9, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Li, Fotinos, Wu, Chen, Zhu, Baranov, Tu, Zhou, Sinha, Kristal, Wang: "N-acetyl-L-tryptophan delays disease onset and extends survival in an amyotrophic lateral sclerosis transgenic mouse model." in: Neurobiology of disease, Vol. 80, pp. 93-103, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Grip, Lonne-Rahm, Holst, Johansson, Nordlind, Theodorsson, El-Nour: "Substance P alterations in skin and brain of chronically stressed atopic-like mice." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 2, pp. 199-205, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Ramalho, Almeida, Fernandes, Costa, Pirraco, Guardão, Delgado, Moreira, Soares: "Neurokinin-1 receptor, a new modulator of lymphangiogenesis in obese-asthma phenotype." in: Life sciences, Vol. 93, Issue 4, pp. 169-77, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Luo, Li, Zhang, Zheng, Wang, Nie, Zhang, Su: "Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 62, Issue 12, pp. 1053-61, 2013 (Sample species: Guinea Pig). Further details: Western Blotting

Yang, Yan, Zhang, Wang: "Substance P participates in immune-mediated hepatic injury induced by concanavalin A in mice and stimulates cytokine synthesis in Kupffer cells." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 459-464, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Lin, Moore, Jones, McGlashon, Talman: "Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 47, pp. 18608-17, 2013 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Talman, Dragon, Jones, Moore, Lin: "Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat." in: Cellular and molecular neurobiology, Vol. 32, Issue 7, pp. 1119-26, 2012 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Caioli, Curcio, Pieri, Antonini, Marolda, Severini, Zona: "Substance P receptor activation induces downregulation of the AMPA receptor functionality in cortical neurons from a genetic model of Amyotrophic Lateral Sclerosis." in: Neurobiology of disease, Vol. 44, Issue 1, pp. 92-101, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Commons: "Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3)." in: Journal of chemical neuroanatomy, Vol. 38, Issue 4, pp. 273-81, 2009 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Amadoro, Pieri, Ciotti, Carunchio, Canu, Calissano, Zona, Severini: "Substance P provides neuroprotection in cerebellar granule cells through Akt and MAPK/Erk activation: evidence for the involvement of the delayed rectifier potassium current." in: Neuropharmacology, Vol. 52, Issue 6, pp. 1366-77, 2007 (Sample species: Rat (Rattus)).

Broccardo, Linari, Agostini, Amadoro, Carpino, Ciotti, Petrella, Petrozza, Severini, Improta: "Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 3, pp. G518-24, 2006 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Broccardo, Ciotti, Linari, Agostini, Petrella, Amadoro, Severini, Improta: "Immunocytochemical distribution of NK-1 and NK-3 tachykinin receptors in isolated pancreatic acini of guinea pigs and rats." in: Peptides, Vol. 26, Issue 11, pp. 2351-4, 2005

Commons, Connolley, Valentino: "A neurochemically distinct dorsal raphe-limbic circuit with a potential role in affective disorders." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 28, Issue 2, pp. 206-15, 2003 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Mounir, Parent: "The expression of neurokinin-1 receptor at striatal and pallidal levels in normal human brain." in: Neuroscience research, Vol. 44, Issue 1, pp. 71-81, 2002 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

Ma, Huang, Chien, Wu, Chen: "Temporal decrease in renal sensory responses in rats after chronic ligation of the bile duct." in: American journal of physiology. Renal physiology, Vol. 283, Issue 1, pp. F164-72, 2002 (Sample species: Rat (Rattus)). Further details: Western Blotting

Kc, Haxhiu, Tolentino-Silva, Wu, Trouth, Mack: "Paraventricular vasopressin-containing neurons project to brain stem and spinal cord respiratory-related sites." in: Respiratory physiology & neurobiology, Vol. 133, Issue 1-2, pp. 75-88, 2002

Yip, Chahl: "Localization of NK1 and NK3 receptors in guinea-pig brain." in: Regulatory peptides, Vol. 98, Issue 1-2, pp. 55-62, 2001 (Sample species: Guinea Pig). Further details: Immunohistochemistry

Anderson, Seybold: "Phosphorylated cAMP response element binding protein increases in neurokinin-1 receptor-immunoreactive neurons in rat spinal cord in response to formalin-induced nociception." in: Neuroscience letters, Vol. 283, Issue 1, pp. 29-32, 2000 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Images

Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Handling References for anti-TACR1 antibody (ABIN152451) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (ABIN152451) in ...
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN268373] -...
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN268373] -...
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunohistochemistry: Neurokinin 1 Receptor Antibody [ABIN268373] - Immunohistochemic...