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CUT&RUN Combo: Beads, Controls, Primary, MNase in One go!

Written/Edited by Julian Pampel, BSc

CUT&RUN stands for "Cleavage Under Targets and Release Using Nuclease". The technique developed from the Henikoff Lab offers a new approach to pursue epigenetics. CUT&RUN introduces some major modifications to eliminate shortcomings inherent to ChIP-seq. It is simple to perform and inherently robust, with extremely low backgrounds, while requiring only ~1/10th the sequencing depth as ChIP. As a result, less sample material is needed, making CUT&RUN a cost-effective option for transcription factor and chromatin profiling.

Assemble Your CUT&RUN Combo

antibodies-online has a special offer for our CUT&RUN customers: A customizable CUT&RUN set, the CUT&RUN Combo. The CUT&RUN Combo gives you a head start in CUT&RUN with concanavalin A beads, a wide selection of antibodies validated for CUT&RUN, control antibodies, pAG-MNase and more, all combined in one set for at least 50 reactions. Benefit from antibodies-online’s expertise - our broad offer of validated CUT&RUN antibodies satisfy the highest standards.

Do you want to perform CUT&RUN experiments but have trouble finding the right reagents? Follow the instructions below to get your customized CUT&RUN Combo for only 600 € in total. Get started now!

  • Browse our list of CUT&RUN Combo components down below and select:
    • Concanavalin A beads
    • Primary Antibody
    • Control Antibodies
    • pAG-MNase
  • Select products for your personal experimental needs
  • Get in touch with our team
  • Get your personalized CUT&RUN Combo for 600 € *
*Your primary is not in the list? Contact our team to discuss options outside this selection

Customize Your Combo

* Required field

Pick Your CUT&RUN Components

CUT&RUN Combo
Overview of Components needed in a CUT&RUN Experiment: 1 Concanavalin A Beads | 2 Controls| 3 Antibodies | 4 pAG-MNase

Concanavalin A beads are responsible for cell immobilization. The unique saccharide-binding properties of plant lectins, such as Concanavalin A have made them suitable for isolation of glycan-presenting cells. For CUT&RUN Magnetic ConA Beads (Agarose) offer easier washing and resuspension steps, while reducing the risk of samples drying out. The negative control antibody is used to establish a reference background for peak calling. This is necessary because of the sparse background signal in CUT&RUN samples compared to ChIP-seq samples. On the other hand, the positive control indicates nonspecific binding of the antibody directed against the protein of interest to other proteins in the sample and is useful to avoid identification of false positive signals. The antibody of interest is determined by the target you want to investigate. For guidance, browse our tables organized by different research focuses such as histone modification or transcription regulation down below. Last, is the pAG-MNase. This enzyme construct of protein A and G is fused to micrococcal nuclease and responsible for DNA digestion prior to chromatin release.

  • Product
    Application
    Cat. No.
    Validations
    Quantity
    Datasheet
    Application CUT&RUN, CUT&Tag, Sep
    Cat. No. ABIN6952467
    Validations
    • (1)
    • (3)
    • (1)
    Quantity 250 μL
    Datasheet Datasheet
    Application CUT&RUN, CUT&Tag, Sep
    Cat. No. ABIN6923139
    Validations
    Quantity 260 μL
    Datasheet Datasheet
  • Positive Control

    Product
    Source
    Reactivity
    Application
    Cat. No.
    Validations
    Quantity
    Datasheet
    Source Guinea Pig
    Reactivity Rabbit
    Application ELISA, IHC, WB, CUT&Tag, CUT&RUN
    Cat. No. ABIN101961
    Validations
    • (105)
    • (3)
    • (1)
    Quantity 200 μL
    Datasheet Datasheet
    Source Rabbit
    Reactivity Mouse
    Application ELISA, IHC, WB, CUT&Tag, CUT&RUN
    Cat. No. ABIN101785
    Validations
    • (4)
    • (1)
    Quantity 2 mg
    Datasheet Datasheet

    Negative Control

    Product
    Source
    Reactivity
    Application
    Cat. No.
    Validations
    Quantity
    Datasheet
    Source Rabbit
    Reactivity Human
    Application WB, IF, ChIP, IP, ChIP-seq, CUT&RUN
    Cat. No. ABIN3023251
    Validations
    • (4)
    • (21)
    • (1)
    Quantity 100 μL
    Datasheet Datasheet
    Source
    Reactivity
    Application CUT&RUN, CUT&Tag
    Cat. No. ABIN6923144
    Validations
    • (1)
    • (1)
    Quantity 135 μL
    Datasheet Datasheet
  • Better Data
    Image
    Only 1/3 of seq reads required
    Less Signal Noise
    Image
    Easer Peak-Calling, Higher Reproducibility
    Less Sample
    Image
    10x less sample in comparison to ChIP-seq
    Optimized Protocol
    Image
    Obtain purified DNA from Cells within 1 day

    antibodies-online: Paving the Way for CUT&RUN

    The successful execution of CUT&RUN hinges on antibody selection. In order to cut adjacent DNA, the pA/G-MNase fusion protein needs to recognize the targeted chromatin part. As is the case with ChIP, the success depends in large part on the affinity of the antibody for its target and specificity under the conditions used for binding.

    We Help Scientists Find the Right Antibody

    Benefit from antibodies-online years of expertise. Since the development of the CUT&RUN technique at the Henikoff Lab we aim to improve the technique. We share our experience with our customers and guide them to find the right antibody.

    antibodies-online is the leading vendor-independent marketplace for research reagents, especially antibodies and ELISA kits. The company was founded in 2006 as a spin-off from RWTH Aachen University (Germany). We deliver research products to customers in over 70 different countries. We currently list over 1,95 million research products, which we deliver to universities, biotech and pharmaceutical companies worldwide.

    Our diverse team consists of highly motivated biologists, IT- and business specialists. Together we strive to provide scientists with the best possible support for their research.

Julian Pampel
Julian Pampel, BSc
Content Manager at antibodies-online.com

Creative mind of antibodies-online with a keen eye for details. Proficient in the field of life-science with a passion for plant biotechnology and clinical study design. Responsible for illustrated and written content at antibodies-online as well as supervision of the antibodies-online scholarship program.

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