anti-Human Calcyclin Binding Protein antibody for ELISA

Recommended Calcyclin Binding Protein Antibody (supplied by: Log in to see )

Antigen
Calcyclin Binding Protein (CACYBP) Antibodies
  • zgc:76993
  • CACYBP
  • sip
  • gig5
  • pnas-107
  • s100a6bp
  • T1P2.12
  • T1P2_12
  • GIG5
  • RP1-102G20.6
  • S100A6BP
  • SIP
  • calcyclin binding protein
  • calcyclin binding protein, putative
  • Calcyclin binding protein, putative
  • SGS domain-containing protein
  • calcyclin binding protein L homeolog
  • cacybp
  • CACYBP
  • PB000926.02.0
  • PCHAS_145490
  • PVX_100855
  • PKH_145680
  • AT1G30070
  • cacybp.L
  • Cacybp
Epitope
AA 1-229
22
14
6
6
5
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
117
54
52
5
4
4
4
3
2
2
1
1
Host
Mouse
77
40
Clonality
Monoclonal
Conjugate
Un-conjugated
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
88
40
26
18
15
15
15
8
7
3
3
3
2
2
1
1
Options
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Catalog No. ABIN393386
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.383331 ABIN565329 PLA ELISA WB Mouse IgG1 kappa AA 1-228, full length Log in to see 2E3 0
11.383331 ABIN5326800 ELISA WB Mouse IgG1 kappa AA 1-228, full length Log in to see 2E3 0
1 ABIN4286808 ELISA WB Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN3187896 ELISA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2463420 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN5956267 ELISA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2591419 ELISA WB Mouse IgG1, kappa AA 1-229 Log in to see 0
1 ABIN4257716 ELISA WB DyLight 488 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257721 ELISA WB DyLight 550 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257713 ELISA WB DyLight 650 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257715 ELISA WB DyLight 680 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257718 ELISA WB DyLight 755 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257714 ELISA FITC Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257707 ELISA WB Alexa Fluor 405 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257708 ELISA WB Alexa Fluor 488 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257709 ELISA WB Alexa Fluor 647 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257710 ELISA WB Alexa Fluor 700 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257712 ELISA WB Biotin Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257722 ELISA WB DyLight 350 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0
1 ABIN4257723 ELISA WB DyLight 405 Mouse IgG2a kappa AA 1-185 Log in to see 3G8 0

General

Antigen Calcyclin Binding Protein (CACYBP) Antibodies
Epitope AA 1-229
(22), (14), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(117), (54), (52), (5), (4), (4), (4), (3), (2), (2), (1), (1)
Host Mouse
(77), (40)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(88), (40), (26), (18), (15), (15), (15), (8), (7), (3), (3), (3), (2), (2), (1), (1)
Pubmed 5 references available
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Product details

Target Details Application Details Handling References Images
Specificity CACYBP (AAH05975, 1 a.a. ~ 229 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: CACYBP (AAH05975, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 2E3
Isotype IgG1 kappa

Target Details

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Antigen
Alternative Name CACYBP (CACYBP Antibody Abstract)
Background

Other Names: Calcyclin-binding protein, CacyBP, hCacyBP, S100A6-binding protein, Siah-interacting protein, CACYBP, S100A6BP, SIP

Gene Description: calcyclin binding protein

Gene Summary: The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Molecular Weight 26210 DA
Gene ID 27101
NCBI Accession NP_001007215, NP_055227
UniProt Q9HB71
Pathways Response to Growth Hormone Stimulus

Application Details

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Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References

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Background publications

Sowa, Bennett, Gygi, Harper: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009

Chen, Han, Zhai, Zhang, Wang, Li, Huang, Wang, Fan: "Expression and clinical significance of CacyBP/SIP in pancreatic cancer." in: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol. 8, Issue 4-5, pp. 470-7, 2008

Zhai, Shi, Jin, Li, Lu, Chen, Wang, Ding, Wang, Fan: "Expression of calcyclin-binding protein/Siah-1 interacting protein in normal and malignant human tissues: an immunohistochemical survey." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 56, Issue 8, pp. 765-72, 2008

Ning, Sun, Hong, Liang, Liu, Han, Liu, Shi, Li, Gong, Zhang, Chen, Guo, Cheng, Wu, Fan: "Calcyclin-binding protein inhibits proliferation, tumorigenicity, and invasion of gastric cancer." in: Molecular cancer research : MCR, Vol. 5, Issue 12, pp. 1254-62, 2008

Sun, Ning, Liu, Liu, Chen, Han, Zhang, Liang, Wu, Fan: "Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma." in: Biochemical and biophysical research communications, Vol. 356, Issue 4, pp. 864-71, 2007

Images

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Supplier Images
Western Blotting (WB) image for anti-Calcyclin Binding Protein (CACYBP) (AA 1-229) antibody (ABIN393386) Western Blot analysis of CACYBP expression in transfected 293T cell line by CACYBP mo...
Immunohistochemistry (IHC) image for anti-Calcyclin Binding Protein (CACYBP) (AA 1-229) antibody (ABIN393386) anti-Calcyclin Binding Protein (CACYBP) (AA 1-229) antibody (Image 2)
Western Blotting (WB) image for anti-Calcyclin Binding Protein (CACYBP) (AA 1-229) antibody (ABIN393386) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Calcyclin Binding Protein (CACYBP) (AA 1-229) antibody (ABIN393386) Western Blot analysis of CACYBP expression in transfected 293T cell line by CACYBP mo...
ELISA image for anti-Calcyclin Binding Protein (CACYBP) (AA 1-229) antibody (ABIN393386) Detection limit for recombinant GST tagged CACYBP is approximately 1ng/ml as a captur...
 image for anti-Calcyclin Binding Protein (CACYBP) (AA 1-229) antibody (ABIN393386) Proximity Ligation Analysis of protein-protein interactions between SKP1 and CACYBP. ...