anti-Human Nuclear Cap Binding Protein Subunit 1, 80kDa antibody for Enzyme Immunoassay

Recommended Nuclear Cap Binding Protein Subunit 1, 80kDa Antibody

Antigen
Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
  • CBP80
  • CG7035
  • Dmel\\CG7035
  • EG:84H4.3
  • ncbp
  • sto1
  • cbp80
  • AU014645
  • AW538051
  • NCBP
  • Sto1
  • ncbp1
  • ABA HYPERSENSITIVE 1
  • ATCBP80
  • CAP-BINDING PROTEIN 80
  • ENS
  • ENSALADA
  • NUCLEAR CAP-BINDING PROTEIN CBP80
  • T10F5.8
  • T10F5_8
  • cap binding protein 80
  • nuclear cap binding protein subunit 1
  • nuclear cap binding protein subunit 1 S homeolog
  • nuclear cap binding protein subunit 1, 80kDa
  • nuclear cap binding protein subunit 1 L homeolog
  • ARM repeat superfamily protein
  • Nuclear cap-binding protein subunit 1-A
  • Nuclear cap-binding protein subunit 1
  • Cbp80
  • ncbp1
  • ncbp1.S
  • NCBP1
  • Ncbp1
  • ncbp1.L
  • ABH1
  • nbp1a
  • ncbp-1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
76
31
27
5
4
4
3
2
2
2
1
1
1
Host
Rabbit
69
7
Clonality
Polyclonal
Conjugate
Un-conjugated
4
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
60
40
18
8
8
7
4
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Catalog No. ABIN272218
$ 434.50
Plus shipping costs $45.00

General

Antigen Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(76), (31), (27), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(69), (7)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(60), (40), (18), (8), (8), (7), (4)
Supplier

Product details

Target Details Application Details Handling Images
Specificity This antibody detects endogenous levels of CBP80 protein. (region surrounding Arg5)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details

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Antigen
Alternative Name NCBP1 (NCBP1 Antibody Abstract)
Background In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex(CBC). CBC consists of two cap binding proteins CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay(NMD), which eliminates mRNAs, which prematurely terminate translation.CBP80-bound mRNA undergoes a Synonyms: CBP80, NCBP, Nuclear cap-binding protein subunit 1
Molecular Weight approx. 88 kDa
Gene ID 4686
NCBI Accession NP_002477
UniProt Q09161
Pathways Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process

Application Details

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Application Notes ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

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Supplier Images
 image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) antibody (ABIN272218) anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) antibody