anti-Mouse (Murine) Nuclear Cap Binding Protein Subunit 1, 80kDa antibody for Immunocytochemistry

Recommended Nuclear Cap Binding Protein Subunit 1, 80kDa Antibody

Antigen
Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
  • CBP80
  • CG7035
  • Dmel\\CG7035
  • EG:84H4.3
  • ncbp
  • sto1
  • cbp80
  • AU014645
  • AW538051
  • NCBP
  • Sto1
  • ncbp1
  • ABA HYPERSENSITIVE 1
  • ATCBP80
  • CAP-BINDING PROTEIN 80
  • ENS
  • ENSALADA
  • NUCLEAR CAP-BINDING PROTEIN CBP80
  • T10F5.8
  • T10F5_8
  • cap binding protein 80
  • nuclear cap binding protein subunit 1
  • nuclear cap binding protein subunit 1 S homeolog
  • nuclear cap binding protein subunit 1, 80kDa
  • nuclear cap binding protein subunit 1 L homeolog
  • ARM repeat superfamily protein
  • Nuclear cap-binding protein subunit 1-A
  • Nuclear cap-binding protein subunit 1
  • Cbp80
  • ncbp1
  • ncbp1.S
  • NCBP1
  • Ncbp1
  • ncbp1.L
  • ABH1
  • nbp1a
  • ncbp-1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
76
31
27
5
4
4
3
2
2
2
1
1
1
Host
Rabbit
69
7
Clonality
Polyclonal
Conjugate
Un-conjugated
4
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
60
39
18
8
7
6
4
1
Options

Available images

Catalog No. ABIN6257658
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN1916284 ELISA ICC IHC IHC (p) WB Rabbit IgG Internal Region Polyclonal 0
1 ABIN5690069 ELISA ICC WB Rabbit IgG AA 420-470 Polyclonal 0
1 ABIN2861145 ICC ELISA WB Rabbit Polyclonal 0
1 ABIN2963535 ICC IHC (p) ELISA WB Rabbit IgG Internal Region Polyclonal 0
-4.16483 ABIN4338250 ICC IF IHC IHC (p) WB Rabbit IgG N-Term Polyclonal 0
-7.16483 ABIN4338249 ELISA ICC IF WB Rabbit Center, all Isoforms Polyclonal 0

General

Antigen Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(76), (31), (27), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(69), (7)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(60), (39), (18), (8), (7), (6), (4), (1)
Supplier

Product details

Target Details Application Details Handling Images
Specificity NCBP1 Antibody detects endogenous levels of total NCBP1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide
Isotype IgG

Target Details

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Antigen
Alternative Name NCBP1 (NCBP1 Antibody Abstract)
Background

Description: Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).

Gene: NCBP1

Molecular Weight 80 kDa
Gene ID 4686
UniProt Q09161
Pathways Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process

Application Details

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Application Notes WB 1:500~1:1000, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

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Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) antibody (ABIN6257658) ABIN6274323 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeab...
Western Blotting (WB) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) antibody (ABIN6257658) Western blot analysis of extracts from HT-29 cells using NCBP1 antibody.The lane on t...