anti-Rabbit BDNF antibody for Western Blotting

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • ANON2
  • BULN2
  • bdnf-A
  • BDNF
  • bdnf
  • brain derived neurotrophic factor
  • brain-derived neurotrophic factor
  • brain-derived neurotrophic factor L homeolog
  • brain-derived neurotrophic factor S homeolog
  • BDNF
  • Bdnf
  • bdnf
  • bdnf.L
  • bdnf.S
Epitope
Middle Region
47
21
14
8
7
7
6
6
6
5
5
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
306
144
126
24
16
16
15
7
7
7
6
6
5
3
3
2
Host
Rabbit
265
73
13
9
9
6
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
35
22
13
5
5
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
301
132
126
48
45
42
38
34
22
13
9
4
3
2
2
2
1
1
1
1
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Catalog No. ABIN2777093
$ 319.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.3156 ABIN2777092 WB Rabbit N-Term Log in to see Polyclonal 1
7.8156 ABIN5013241 ICC IHC WB Rabbit IgG AA 20-252 Log in to see Polyclonal 0
7.8156 ABIN6001328 IF/ICC IHC IP WB Rabbit AA 20-252 Log in to see Polyclonal 0
4.8156 ABIN1858131 ICC IHC (fro) IHC (p) ELISA WB Guinea Pig IgG AA 16-183 Log in to see Polyclonal 0
4.8156 ABIN6002559 IF/ICC IHC IP WB Mouse IgG2b kappa AA 20-252 Log in to see 0
4.8156 ABIN6002565 IF/ICC IHC IP WB Mouse AA 20-252 Log in to see 0
4.8156 ABIN5662140 WB Mouse IgG2b kappa AA 20-252 Log in to see C4 0
4.8156 ABIN5662314 WB Mouse AA 20-252 Log in to see 0
4.8156 ABIN2901111 ELISA IF/ICC IHC WB Guinea Pig AA 16-183 Log in to see Polyclonal 0
1 ABIN2617531 WB Biotin Guinea Pig IgG AA 16-183 Log in to see Polyclonal 0
1 ABIN2617537 WB FITC Guinea Pig IgG AA 16-183 Log in to see Polyclonal 0
1 ABIN2590118 WB Guinea Pig IgG AA 16-183 Log in to see Polyclonal 0
1 ABIN326619 IHC IHC (fro) WB Rabbit AA 69-82 Log in to see Polyclonal 0
1 ABIN1834607 WB Rabbit AA 150-199 Log in to see Polyclonal 0
1 ABIN6055817 IF/ICC IHC WB Biotin Mouse IgG Log in to see 0
1 ABIN6029340 IF/ICC IHC IP WB APC Guinea Pig Log in to see Polyclonal 0

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Epitope Middle Region
(47), (21), (14), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(306), (144), (126), (24), (16), (16), (15), (7), (7), (7), (6), (6), (5), (3), (3), (2)
Host Rabbit
(265), (73), (13), (9), (9), (6)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(35), (22), (13), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(301), (132), (126), (48), (45), (42), (38), (34), (22), (13), (9), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling References for anti-BDNF antibody (ABIN2777093) Images
Blocking Peptide Blocking peptide for this product available: ABIN973798
Sequence EWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG
Predicted Reactivity Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against BDNF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human BDNF
Plasmids, Primers & others

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling References for anti-BDNF antibody (ABIN2777093) Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression BDNF is reduced in both Alzheimer's and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders.The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
Alias Symbols: MGC34632
Protein Interaction Partner: UBC, AGO3, INPP5K, F11R, JUNB, APP, ELAVL1, Ntrk2, CADPS2, MBTPS1, SORT1, NOS3, NTF4, NTF3, ESR1, NCAM1, CPE, BDNF,
Protein Size: 247
Molecular Weight 27 kDa
Gene ID 627
NCBI Accession NM_001709, NP_001700
UniProt P23560
Pathways RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling References for anti-BDNF antibody (ABIN2777093) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 247 AA

Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details References for anti-BDNF antibody (ABIN2777093) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BDNF antibody (ABIN2777093)

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling Images back to top
Product cited in:

McCarthy, Mueller, Cannon, Huizenga, Darnell, Bhide, Sadri-Vakili: "Prenatal Cocaine Exposure Alters BDNF-TrkB Signaling in the Embryonic and Adult Brain." in: Developmental neuroscience, Vol. 38, Issue 5, pp. 365-374, 2017

McCarthy, Bell, Cannon, Mueller, Huizenga, Sadri-Vakili, Fadool, Bhide: "Reversal Learning Deficits Associated with Increased Frontal Cortical Brain-Derived Neurotrophic Factor Tyrosine Kinase B Signaling in a Prenatal Cocaine Exposure Mouse Model." in: Developmental neuroscience, Vol. 38, Issue 5, pp. 354-364, 2016

Kight, McCarthy: "Sex differences and estrogen regulation of BDNF gene expression, but not propeptide content, in the developing hippocampus." in: Journal of neuroscience research, Vol. 95, Issue 1-2, pp. 345-354, 2016

Kasemeier-Kulesa, Morrison, Lefcort, Kulesa: "TrkB/BDNF signalling patterns the sympathetic nervous system." in: Nature communications, Vol. 6, pp. 8281, 2015

Vassoler, White, Schmidt, Sadri-Vakili, Pierce: "Epigenetic inheritance of a cocaine-resistance phenotype." in: Nature neuroscience, Vol. 16, Issue 1, pp. 42-7, 2012

McCarthy, Zhang, Darnell, Sangrey, Yanagawa, Sadri-Vakili, Bhide: "Cocaine alters BDNF expression and neuronal migration in the embryonic mouse forebrain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 38, pp. 13400-11, 2011

Background publications

Hashimoto, Moriguchi, Yamashita, Mori, Nemoto, Okada, Hori, Noguchi, Kunugi, Ohnishi: "Dose-dependent effect of the Val66Met polymorphism of the brain-derived neurotrophic factor gene on memory-related hippocampal activity." in: Neuroscience research, Vol. 61, Issue 4, pp. 360-7, 2008

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling References for anti-BDNF antibody (ABIN2777093) back to top
Supplier Images
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) WB Suggested Anti-BDNF Antibody Titration: 0.2-1 ug/ml Positive Control: Human Liver
Immunohistochemistry (IHC) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Sample Type : Rhesus macaque spinal cord Primary Antibody Dilution : 1:300 Second...
Immunohistochemistry (IHC) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (Image 3)
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Host: Mouse Target Name: BDNF Sample Tissue: Mouse Testis Antibody Dilution: 1...
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Host: Rabbit Target Name: BDNF Sample Type: ACHN Whole Cell lysates Antibody Di...
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Host: Rabbit Target Name: BDNF Sample Tissue: Human Ovary Tumor Antibody Diluti...
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Host: Mouse Target Name: BDNF Sample Tissue: Mouse Brain Antibody Dilution: 1u...
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Host: Mouse Target Name: BDNF Sample Tissue: Mouse Pancreas Antibody Dilution: ...
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Host: Rabbit Target Name: BDNF Sample Type: Fetal Liver lysates Antibody Diluti...
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (ABIN2777093) Host: Rabbit Target Name: BDNF Sample Tissue: Human ACHN Antibody Dilution: 1....