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|Antigen||Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies|
|Conjugate||This FGF2 antibody is un-conjugated Alternatives|
Functional Studies (Func), Western Blotting (WB)
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Product Details anti-FGF2 AntibodyTarget Details FGF2 Application Details Handling Images
|Specificity||The antibody will detect FGF-2 (basic).|
|Purification||Protein A affinity chromatography|
|Immunogen||Highly pure (> 98%) recombinant human FGF-2 (basic)|
Target Details FGF2Product Details anti-FGF2 Antibody Application Details Handling Images back to top
|Alternative Name||FGF Basic / FGF2 (FGF2 Antibody Abstract)|
|Background||FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus|
Application DetailsProduct Details anti-FGF2 Antibody Target Details FGF2 Handling Images back to top
Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human FGF-2 basic(5 ng/mL) on HUVEC cells, a concentration of about 3.5 μg/mL of this antibody is required. Western Blot: To detect human FGF-2 basic by Western blot this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant human FGF-2 (basic) is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF2 Antibody Target Details FGF2 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of > / = 0.2 mg/mL.|
|Buffer||PBS, pH 7.4, without stabilizer or preservative|
|Storage||4 °C/-20 °C|
|Storage Comment||The lyophilized antibody is stable at room temperature for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > / = 0.2 mg/ml the antibody is stable for six weeks at 2-8 °C.|
|Expiry Date||12 months|