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|Antigen||Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies|
|Conjugate||This FGF2 antibody is conjugated to Biotin Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
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Product Details anti-FGF2 AntibodyTarget Details FGF2 Application Details Handling Images
|Specificity||Recognizes human basic FGF. Does not recognize human acidic FGF or a panel of other human cytokines.|
|Characteristics||Synonyms: FGFB, Heparin-binding growth factor 2, Fibroblast growth factor 2 (basic), BFGF, HBGF-2,HBGF2|
|Purification||Protein G Chromatography|
|Purity||> 95 % by SDS-PAGE|
Target Details FGF2Product Details anti-FGF2 Antibody Application Details Handling Images back to top
|Alternative Name||FGF Basic / FGF2 (FGF2 Antibody Abstract)|
|Background||FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus|
Application DetailsProduct Details anti-FGF2 Antibody Target Details FGF2 Handling Images back to top
ELISA: This antibody can be used as a solution phase antibody at 2 μg/mL in a two site ELISA withunconjugated bFGF antibody (Cat. No. ABIN264366). Typically the ELISA has a workingrange of 0-2000 pg/mL and a sensitivity of 5 pg/mL with the appropriate standard. Recommended sample volumes are 25-200 l. Recovery of human basic FBF is human serum is typically >85 % in the ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF2 Antibody Target Details FGF2 Application Details Images back to top
|Buffer||PBS containing 0.08 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|