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|Antigen||Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies|
|Conjugate||This FGF2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-FGF2 AntibodyTarget Details FGF2 Application Details Handling Images
E.coli derived recombinant Human FGF-basic.
Type of Immunogen: Cell extract
Target Details FGF2Product Details anti-FGF2 Antibody Application Details Handling Images back to top
|Alternative Name||FGF2 / BFGF (FGF2 Antibody Abstract)|
Name/Gene ID: FGF2
Synonyms: FGF2, Basic fibroblast growth factor, FGFB, Fibroblast growth factor 2, FGF-2, Prostatropin, BFGF, HBGF-2
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus|
Application DetailsProduct Details anti-FGF2 Antibody Target Details FGF2 Handling Images back to top
Approved: ELISA, IHC-P (5 μg/mL), Neut (0.25 - 0.4 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7426 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (LS-C104313) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF2 Antibody Target Details FGF2 Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling back to top