anti-Human FLT1 antibody for ELISA

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • FLT
  • FLT-1
  • VEGFR-1
  • VEGFR1
  • FLT1
  • flt1b
  • sFlt1
  • AI323757
  • Flt-1
  • fms related tyrosine kinase 1
  • FMS-related tyrosine kinase 1
  • vascular endothelial growth factor receptor 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
  • FMS-like tyrosine kinase 1
  • FLT1
  • Flt1
  • CpipJ_CPIJ012087
  • flt1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
435
148
112
17
17
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
253
248
13
5
3
1
Clonality
Polyclonal
Conjugate
This FLT1 antibody is un-conjugated
35
20
10
10
10
9
9
7
7
7
6
6
6
6
6
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
305
195
149
102
101
76
60
39
22
13
13
9
9
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN6265971
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.278215 ABIN969146 ELISA WB Mouse IgG1 Log in to see 3D10 2
18.778215 ABIN3184639 ELISA IHC Rabbit IgG Ser385 Log in to see Polyclonal 0
18.778215 ABIN3182936 ELISA IHC Rabbit IgG pTyr1048 Log in to see Polyclonal 0
17.278215 ABIN966154 ELISA WB Mouse IgG1 Extracellular Log in to see 3D10 2
15.778215 ABIN3183067 ELISA WB Rabbit IgG pTyr1213 Log in to see Polyclonal 0
15.778215 ABIN3184640 ELISA IHC Rabbit IgG Ser386 Log in to see Polyclonal 0
15.778215 ABIN3182563 ELISA IHC WB Rabbit IgG pTyr1333 Log in to see Polyclonal 0
15.778215 ABIN931310 ELISA WB Mouse IgG1 Log in to see 3D10 0
13 ABIN6256626 ELISA ICC IF WB Rabbit IgG pTyr1213 Log in to see Polyclonal 0
12.778215 ABIN4901545 ELISA WB Mouse IgG1 Log in to see 3D10 0
12.778215 ABIN5621475 ELISA IHC Rabbit IgG total Log in to see Polyclonal 0
12.778215 ABIN5621474 ELISA IHC WB Rabbit IgG pTyr1333 Log in to see Polyclonal 0
12.778215 ABIN2745579 ELISA WB Mouse IgG1 Log in to see EWC 0
12.778215 ABIN2745580 IP ELISA WB Mouse IgG1 Log in to see EWF 0
12.778215 ABIN284423 FACS IF IHC ELISA WB Mouse IgG1 Log in to see FLT12 0
12.778215 ABIN2154566 IHC ELISA Rabbit IgG pTyr1048 Log in to see Polyclonal 0
12.778215 ABIN2142003 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.778215 ABIN2154567 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
12.778215 ABIN2154559 IP ELISA WB Mouse IgG1 Log in to see EWC 0
12.778215 ABIN2154561 IP ELISA WB Mouse IgG1 Log in to see EWF 0

General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(435), (148), (112), (17), (17), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(253), (248), (13), (5), (3), (1)
Clonality
Polyclonal
Conjugate This FLT1 antibody is un-conjugated
(35), (20), (10), (10), (10), (9), (9), (7), (7), (7), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(305), (195), (149), (102), (101), (76), (60), (39), (22), (13), (13), (9), (9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN6265971) Images
Specificity VEGFR1 Antibody detects endogenous levels of total VEGFR1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human VEGFR1
Isotype IgG
Plasmids, Primers & others

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling References for anti-FLT1 antibody (ABIN6265971) Images back to top
Antigen
Alternative Name VEGFR1 (FLT1 Antibody Abstract)
Background

Description: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity, may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain, it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.

Gene: FLT1

Molecular Weight 180 kDa
Gene ID 2321
UniProt P17948
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling References for anti-FLT1 antibody (ABIN6265971) Images back to top
Application Notes WB 1:500-1:2000, IHC-f 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details References for anti-FLT1 antibody (ABIN6265971) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-FLT1 antibody (ABIN6265971)

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling Images back to top
Product cited in:

Ai, Zhang, Gao, Qin, Zhong, Gu, Li, Qiao, Tian, Cui, Yang, Bi, Xiao, Chen, Liu, Zhou, Sun, Yang: "Sesquiterpene binding Gly-Leu-Ser/Lys-"co-adaptation pocket" to inhibit lung cancer cell epithelial-mesenchymal transition." in: Oncotarget, Vol. 8, Issue 41, pp. 70192-70203, 2018

Meng, Chen, Lei, Han, Zhong, Wang, Liu, Gao, Zhang, Tan, Liu, Zhou, Sun, Yang: "Hsp90β promotes aggressive vasculogenic mimicry via epithelial-mesenchymal transition in hepatocellular carcinoma." in: Oncogene, 2018

Images

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN6265971) back to top
Supplier Images
Western Blotting (WB) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody (ABIN6265971) Western blot analysis of VEGFR1 expression in mouse brain and rat spleen tissue lysat...
Western Blotting (WB) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody (ABIN6265971) Western blot analysis of VEGFR1 expression in HeLa whole cell lysates,The lane on the...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody (ABIN6265971) ABIN6269175 staining A-431  cells by IF/ICC. The sample were fixed with PFA and perme...
Immunohistochemistry (IHC) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody (ABIN6265971) ABIN6269175 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissu...
Immunohistochemistry (IHC) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody (ABIN6265971) ABIN6269175 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissu...