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|Antigen||Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) Antibodies|
|Epitope||AA 50-150 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This NTRK3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
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Product Details anti-NTRK3 AntibodyTarget Details NTRK3 Application Details Handling Images
|Immunogen||A synthetic peptide made to the extracellular domain of the human TrkC protein (within residues 50-150). [UniProt Q16288].|
|Plasmids, Primers & others|
Target Details NTRK3Product Details anti-NTRK3 Antibody Application Details Handling Images back to top
|Alternative Name||TrkC (NTRK3 Antibody Abstract)|
|Background||Gene Symbol: NTRK3|
|UniProt||Q16288, Q6VNS1, Q03351|
|Pathways||RTK Signaling, Neurotrophin Signaling Pathway, Regulation of Cell Size|
Application DetailsProduct Details anti-NTRK3 Antibody Target Details NTRK3 Handling Images back to top
|Application Notes||Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry-Paraffin 1:200This TrkC antibody is useful for Immunohistochemistry-Paraffin and Immunocytochemistry/ Immunofluorescence.In ICC/IF, membrane staining was observed in neuro2a cells. In IHC-P, staining was observed in the membrane of mouse brain and spinal cord. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Immunocytochemistry Protocol specific for TrkC antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry Protocol specific for TrkC antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NTRK3 Antibody Target Details NTRK3 Application Details Images back to top
|Reconstitution||Reconstitute with 0.1 mL sterilized water to desired concentration. Centrifuge to remove any insoluble material.|
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
ImagesProduct Details anti-NTRK3 Antibody Target Details NTRK3 Application Details Handling back to top