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|Antigen||SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies|
|Reactivity||Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SHC1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-SHC1 AntibodyTarget Details SHC1 Application Details Handling References for anti-SHC1 antibody (ABIN967732) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details SHC1Product Details anti-SHC1 Antibody Application Details Handling References for anti-SHC1 antibody (ABIN967732) Images back to top
|Alternative Name||SHC (SHC1 Antibody Abstract)|
|Background||The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway. To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs. Phosphorylated SHC forms a complex with the adaptor protein GRB2. Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane. Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange. Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases. The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.|
|Molecular Weight||66/52/46 kDa|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-SHC1 Antibody Target Details SHC1 Handling References for anti-SHC1 antibody (ABIN967732) Images back to top
Related Products: ABIN968587, ABIN967390
|Restrictions||For Research Use only|
HandlingProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details References for anti-SHC1 antibody (ABIN967732) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
References for anti-SHC1 antibody (ABIN967732)Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling Images back to top
|Product cited in:||
Zubiaur, Fernández, Ferrero, Salmerón, Malissen, Malavasi, Sancho: "CD38 is associated with lipid rafts and upon receptor stimulation leads to Akt/protein kinase B and Erk activation in the absence of the CD3-zeta immune receptor tyrosine-based activation motifs." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 13-22, 2002
Webb, Thomas, Gonias: "Plasminogen activator inhibitor 1 functions as a urokinase response modifier at the level of cell signaling and thereby promotes MCF-7 cell growth." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 741-52, 2001
Conti, Sipione, Magrassi, Bonfanti, Rigamonti, Pettirossi, Peschanski, Haddad, Pelicci, Milanesi, Pelicci, Cattaneo: "Shc signaling in differentiating neural progenitor cells." in: Nature neuroscience, Vol. 4, Issue 6, pp. 579-86, 2001
Cook, McCormick: "Inhibition by cAMP of Ras-dependent activation of Raf." in: Science (New York, N.Y.), Vol. 262, Issue 5136, pp. 1069-72, 1993
Cutler, Liu, Damen, Krystal: "Multiple cytokines induce the tyrosine phosphorylation of Shc and its association with Grb2 in hemopoietic cells." in: The Journal of biological chemistry, Vol. 268, Issue 29, pp. 21463-5, 1993
ImagesProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling References for anti-SHC1 antibody (ABIN967732) back to top