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|Antigen||tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This TIE1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-TIE1 AntibodyTarget Details TIE1 Application Details Handling References for anti-TIE1 antibody (ABIN359947) Images
|Specificity||This antibody detects TIE1 at N-term.|
|Purification||Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human TIE.|
Target Details TIE1Product Details anti-TIE1 Antibody Application Details Handling References for anti-TIE1 antibody (ABIN359947) Images back to top
|Alternative Name||TIE1 (TIE1 Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1|
|Molecular Weight||125089 Da|
|Gene ID||7075, 9606|
Application DetailsProduct Details anti-TIE1 Antibody Target Details TIE1 Handling References for anti-TIE1 antibody (ABIN359947) Images back to top
ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TIE1 Antibody Target Details TIE1 Application Details References for anti-TIE1 antibody (ABIN359947) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-TIE1 antibody (ABIN359947)Product Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling Images back to top
Verstovsek, Kantarjian, Manshouri, OBrien, Faderl, Talpaz, Cortes, Albitar: "Prognostic significance of Tie-1 protein expression in patients with early chronic phase chronic myeloid leukemia." in: Cancer, Vol. 94, Issue 5, pp. 1517-21, 2002
Tsiamis, Morris, Marron, Brindle: "Vascular endothelial growth factor modulates the Tie-2:Tie-1 receptor complex." in: Microvascular research, Vol. 63, Issue 2, pp. 149-58, 2002
Sato, Tozawa, Deutsch, Wolburg-Buchholz, Fujiwara, Gendron-Maguire, Gridley, Wolburg, Risau, Qin: "Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation." in: Nature, Vol. 376, Issue 6535, pp. 70-4, 1995
Partanen, Armstrong, Mäkelä, Korhonen, Sandberg, Renkonen, Knuutila, Huebner, Alitalo: "A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains." in: Molecular and cellular biology, Vol. 12, Issue 4, pp. 1698-707, 1992
ImagesProduct Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling References for anti-TIE1 antibody (ABIN359947) back to top