We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling Images
|Specificity||The antibody will react with all VEGF-A isoforms.|
|Purification||Affinity Chromatography with immobilized Protein A|
|Immunogen||Highly purified N-terminal 20 amino acid peptide of Human VEGF-A|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling Images back to top
|Alternative Name||VEGF-A (VEGFA Antibody Abstract)|
|Background||VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
ELISA: To detect Human VEGF by Direct ELISA a concentration of at least 0.5-2 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 1,0- 2,5 ng/well of recombinant Human VEGF165 or other VEGF splice forms. Western Blot: 5-10 μg/mL. It will detect 25 ng/lane of recombinant human and murineVEGF-A under reducing conditions, dimers are detected at higher protein concentrations.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of > 0.5 mg/mL|
|Buffer||PBS, pH 7.4 containing no preservative|
|Storage||4 °C/-20 °C|
|Storage Comment||The lyophilized IgG is stable at 2-8 °C for at least one month and for longer when kept at -20 °C. When reconstituted the antibody is stable for at least six weeks at 2-4 °C.|