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|Antigen||Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies|
|Epitope||AA 454-469, Middle Region Alternatives|
|Conjugate||This VEGFR2 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|6 references available|
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Product Details anti-VEGFR2 AntibodyTarget Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN3044301) Images
|Purpose||Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 2(KDR) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 2(KDR) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Gene Name: kinase insert domain receptor(a type III receptor tyrosine kinase)
Protein Name: Vascular endothelial growth factor receptor 2
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2(454-469aa HHIHWYWQLEEECANE).|
|Plasmids, Primers & others|
Target Details VEGFR2Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 antibody (ABIN3044301) Images back to top
|Alternative Name||KDR (KDR Antibody Abstract)|
KDR(Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al.(1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor(VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1(Flk1) in mouse, is exclusively expressed in endothelial cells(Plate et al., 1993). Flk1 is the mouse homolog of KDR(Matthews et al., 1991).
Synonyms: CD309 antibody|CD309 antigen antibody|Fetal liver kinase 1 antibody|FLK-1 antibody|FLK1 antibody|Kdr antibody|Kinase insert domain receptor(a type III receptor tyrosine kinase) antibody|Kinase insert domain receptor antibody|KRD1 antibody|Ly73 antibody|Protein tyrosine kinase receptor FLK1 antibody|Protein-tyrosine kinase receptor flk-1 antibody|Tyrosine kinase growth factor receptor antibody|Vascular endothelial growth factor receptor 2 antibody|VEGFR 2 antibody|VEGFR antibody|VEGFR-2 antibody|VEGFR2 antibody|VGFR2_HUMAN antibody
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling|
Application DetailsProduct Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 antibody (ABIN3044301) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for KDR is approximately 5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 antibody (ABIN3044301) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
References for anti-VEGFR2 antibody (ABIN3044301)Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
|Product cited in:||
Huang, Li, Pan, Cheng, Ren, Jia, Ma, Xu: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, 2018
Liu, Yang, Zhang, Shui, Song, Yao, Dai, Sun: "Fructopyrano-(1?4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner." in: International journal of clinical and experimental medicine, Vol. 7, Issue 11, pp. 3859-69, 2014
Zhu, Tuerxun, Hui, Abliz: "Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 647-651, 2014
Wang, Liu, Wang, Wang: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." in: Oncology letters, Vol. 6, Issue 1, pp. 175-180, 2013
Wu, You, Ma, Li, Yuan, Li, Ye, Liu, Yao, Chen, Lai, Yang: "Role of transient receptor potential ion channels and evoked levels of neuropeptides in a formaldehyde-induced model of asthma in BALB/c mice." in: PLoS ONE, Vol. 8, Issue 5, pp. e62827, 2013
Sun, Chen, Song, Ren, Wei, Liu, Zhang, Lv: "Effect of visfatin on the function of endothelial progenitor cells in high-fat-fed obese rats and investigation of its mechanism of action." in: International journal of molecular medicine, Vol. 30, Issue 3, pp. 622-8, 2012
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