anti-Human VEGFR2 antibody for Immunohistochemistry (Frozen Sections)

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • CD309
  • FLK1
  • VEGFR
  • VEGFR2
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • sVEGFR-2
  • Vegfr-2
  • FLK-1
  • flk-1
  • vegfr-2
  • KDR
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • kinase insert domain receptor
  • kinase insert domain protein receptor
  • vascular endothelial growth factor receptor kdr-like
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • KDR
  • Kdr
  • LOC100136647
  • kdr
Epitope
AA 454-469, Middle Region
79
61
56
40
27
24
24
22
21
21
19
16
15
15
14
12
12
10
10
9
8
7
7
7
7
6
6
6
6
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
760
413
213
28
14
8
5
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
578
265
72
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
52
39
33
32
23
22
19
13
13
13
12
11
9
9
8
8
8
8
6
6
5
5
5
5
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
587
331
329
297
232
156
142
59
44
31
26
19
17
8
7
7
6
6
5
2
1
1
1
1
Options
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Catalog No. ABIN3044301
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.470581 ABIN449648 ICC IHC (fro) IF IHC ELISA WB Mouse IgG1 Log in to see 20I6 0
16.470581 ABIN5681628 ELISA FACS IHC (fro) IP WB Rabbit Log in to see Polyclonal 0
13.25679 ABIN285774 IHC (fro) IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
13.25679 ABIN2154574 IHC (fro) WB Mouse IgG1 Extracellular Domain Log in to see FLK-6 0
13.25679 ABIN1689505 IHC (fro) WB Mouse IgG1 Log in to see FLK-6 0
10 ABIN3043643 IHC (fro) Mouse IgG1 Extracellular Domain Log in to see KDR-1 7
10 ABIN3031551 ICC IHC (fro) IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal 0
4 ABIN155179 EIA FACS IHC (fro) IF WB Mouse IgG1 Extracellular Domain Log in to see 4 (20I6) 1
4 ABIN5662036 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see 0
1 ABIN1106250 IHC (fro) WB Mouse IgG1 Extracellular Domain Log in to see FLK-6 2
1 ABIN115682 EIA Func IHC (fro) IP WB Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN155185 EIA Func IHC (fro) IP WB Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN1493515 IHC IHC (fro) WB Mouse IgG1 Extracellular Domain Log in to see FLK-6 0
1 ABIN600313 IHC IHC (fro) IHC (p) WB Mouse IgG1 Extracellular Domain Log in to see 0
1 ABIN2624471 ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 454-469 Log in to see Polyclonal 0
1 ABIN355020 ELISA FACS IHC IHC (fro) Mouse IgG1 Log in to see 0
1 ABIN155178 EIA FACS IHC (fro) IF WB Biotin Mouse IgG1 Extracellular Domain Log in to see 4 (20I6) 0
1 ABIN155161 EIA FACS IHC (fro) WB Biotin Mouse IgG1 Extracellular Domain Log in to see KDREIC 0
1 ABIN115148 EIA FACS IHC (fro) WB Mouse IgG1 Extracellular Domain Log in to see KDREIC 0
1 ABIN2742370 ELISA FACS IF IHC IHC (fro) IP Mouse IgG1, kappa Log in to see KDR721 0

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope AA 454-469, Middle Region
(79), (61), (56), (40), (27), (24), (24), (22), (21), (21), (19), (16), (15), (15), (14), (12), (12), (10), (10), (9), (8), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(760), (413), (213), (28), (14), (8), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(578), (265), (72), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(52), (39), (33), (32), (23), (22), (19), (13), (13), (13), (12), (11), (9), (9), (8), (8), (8), (8), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(587), (331), (329), (297), (232), (156), (142), (59), (44), (31), (26), (19), (17), (8), (7), (7), (6), (6), (5), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN3044301) Images
Purpose Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 2(KDR) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Sequence HHIHWYWQLE EECANE
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 2(KDR) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Gene Name: kinase insert domain receptor(a type III receptor tyrosine kinase)
Protein Name: Vascular endothelial growth factor receptor 2
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2(454-469aa HHIHWYWQLEEECANE).
Isotype IgG
Plasmids, Primers & others

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 antibody (ABIN3044301) Images back to top
Antigen
Alternative Name KDR (KDR Antibody Abstract)
Background KDR(Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al.(1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor(VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1(Flk1) in mouse, is exclusively expressed in endothelial cells(Plate et al., 1993). Flk1 is the mouse homolog of KDR(Matthews et al., 1991).

Synonyms: CD309 antibody|CD309 antigen antibody|Fetal liver kinase 1 antibody|FLK-1 antibody|FLK1 antibody|Kdr antibody|Kinase insert domain receptor(a type III receptor tyrosine kinase) antibody|Kinase insert domain receptor antibody|KRD1 antibody|Ly73 antibody|Protein tyrosine kinase receptor FLK1 antibody|Protein-tyrosine kinase receptor flk-1 antibody|Tyrosine kinase growth factor receptor antibody|Vascular endothelial growth factor receptor 2 antibody|VEGFR 2 antibody|VEGFR antibody|VEGFR-2 antibody|VEGFR2 antibody|VGFR2_HUMAN antibody
UniProt P35968
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 antibody (ABIN3044301) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for KDR is approximately 5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 antibody (ABIN3044301) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-VEGFR2 antibody (ABIN3044301)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Product cited in:

Huang, Li, Pan, Cheng, Ren, Jia, Ma, Xu: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, 2018

Liu, Yang, Zhang, Shui, Song, Yao, Dai, Sun: "Fructopyrano-(1?4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner." in: International journal of clinical and experimental medicine, Vol. 7, Issue 11, pp. 3859-69, 2014

Zhu, Tuerxun, Hui, Abliz: "Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 647-651, 2014

Wang, Liu, Wang, Wang: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." in: Oncology letters, Vol. 6, Issue 1, pp. 175-180, 2013

Wu, You, Ma, Li, Yuan, Li, Ye, Liu, Yao, Chen, Lai, Yang: "Role of transient receptor potential ion channels and evoked levels of neuropeptides in a formaldehyde-induced model of asthma in BALB/c mice." in: PLoS ONE, Vol. 8, Issue 5, pp. e62827, 2013

Sun, Chen, Song, Ren, Wei, Liu, Zhang, Lv: "Effect of visfatin on the function of endothelial progenitor cells in high-fat-fed obese rats and investigation of its mechanism of action." in: International journal of molecular medicine, Vol. 30, Issue 3, pp. 622-8, 2012

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN3044301) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 454-469), (Middle Region) antibody (ABIN3044301) Anti-VEGF Receptor 2 antibody, ICC ICC: HELA Cell
Immunohistochemistry (IHC) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 454-469), (Middle Region) antibody (ABIN3044301) Anti-VEGF Receptor 2 antibody, IHC(F) IHC(F): Human Placenta Tissue
Immunohistochemistry (IHC) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 454-469), (Middle Region) antibody (ABIN3044301) Anti-VEGF Receptor 2 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 454-469), (Middle Region) antibody (ABIN3044301) Anti-VEGF Receptor 2 antibody, Western blotting WB: SMMC Cell Lysate