anti-Human VEGFR2 antibody for Western Blotting

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • CD309
  • FLK1
  • VEGFR
  • VEGFR2
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • sVEGFR-2
  • Vegfr-2
  • FLK-1
  • flk-1
  • vegfr-2
  • KDR
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • kinase insert domain receptor
  • kinase insert domain protein receptor
  • vascular endothelial growth factor receptor kdr-like
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • KDR
  • Kdr
  • LOC100136647
  • kdr
Epitope
AA 29-60, N-Term
62
59
58
42
24
23
21
21
20
16
16
15
15
12
10
10
9
9
8
7
7
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
620
397
206
28
8
5
5
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
555
145
59
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
44
34
28
26
16
12
11
9
6
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
494
270
269
200
133
106
45
39
31
26
25
19
17
8
8
7
7
5
5
2
1
1
1
1
1
Options
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Catalog No. ABIN3043274
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0788064 ABIN3044301 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 454-469, Middle Region Log in to see Polyclonal 3
3.0788064 ABIN152058 IHC (p) IP IHC WB Rabbit C-Term, AA 1158-1365 Log in to see Polyclonal 8
3.0788064 ABIN2792307 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.0788064 ABIN250774 ICC IF WB Rabbit AA 1300-1367, C-Term, Native Form Log in to see Polyclonal 7
3.0788064 ABIN5519021 CC IHC (p) WB Rabbit IgG AA 20-242 Log in to see Polyclonal 0
3.0788064 ABIN152343 CyTOF ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see EIC 1
3.0788064 ABIN2506667 ICC IF IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN449646 CyTOF ELISA FACS WB Mouse IgG1 Log in to see 4H3 0
3.0788064 ABIN783332 WB Rabbit AA 1057-1061 Log in to see Polyclonal 2
3.0788064 ABIN2869451 ELISA FACS ICC WB Mouse IgG1 AA 20-764 Log in to see 4B4 3
3.0788064 ABIN2705327 IC IF IHC WB Rabbit C-Term, pTyr1175 Log in to see Polyclonal 0
3.0788064 ABIN391979 FACS IF IHC (p) WB Rabbit Ig Fraction AA 1326-1356, C-Term Log in to see Polyclonal 4
3.0788064 ABIN359866 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 5
3.0788064 ABIN257630 ICC IF IHC (p) IHC WB Rabbit pTyr951 Log in to see Polyclonal 0
3.0788064 ABIN1531998 IHC ELISA WB Rabbit IgG AA 1141-1190, pTyr1175 Log in to see Polyclonal 1
3.0788064 ABIN1532428 ELISA WB Rabbit IgG AA 1025-1074 Log in to see Polyclonal 1
3.0788064 ABIN1533036 IHC ELISA WB Rabbit IgG AA 1141-1190 Log in to see Polyclonal 1
3.0788064 ABIN1531999 IHC ELISA WB Rabbit IgG AA 1180-1229, pTyr1214 Log in to see Polyclonal 1
3.0788064 ABIN1533037 IHC ELISA WB Rabbit IgG AA 1180-1229 Log in to see Polyclonal 1
3.0788064 ABIN1531411 ELISA WB Rabbit IgG AA 1025-1074, pTyr1059 Log in to see Polyclonal 1

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope AA 29-60, N-Term
(62), (59), (58), (42), (24), (23), (21), (21), (20), (16), (16), (15), (15), (12), (10), (10), (9), (9), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(620), (397), (206), (28), (8), (5), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(555), (145), (59), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(44), (34), (28), (26), (16), (12), (11), (9), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Western Blotting (WB)
(494), (270), (269), (200), (133), (106), (45), (39), (31), (26), (25), (19), (17), (8), (8), (7), (7), (5), (5), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN3043274) Images
Purpose Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 2(KDR) detection. Tested with WB in Human.
Sequence LDLPRLSIQK DILTIKANTT LQITCRGQRD LD
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 2(KDR) detection. Tested with WB in Human.
Gene Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
Protein Name: Vascular endothelial growth factor receptor 2
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human KDR (29-60aa LDLPRLSIQKDILTIKANTTLQITCRGQRDLD), different from the related mouse and rat sequences by three amino acids.
Isotype IgG
Plasmids, Primers & others

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 antibody (ABIN3043274) Images back to top
Antigen
Alternative Name KDR (KDR Antibody Abstract)
Background KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al. (1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells (Plate et al., 1993). Flk1 is the mouse homolog of KDR (Matthews et al., 1991).

Synonyms: CD309 antibody|CD309 antigen antibody|Fetal liver kinase 1 antibody|FLK-1 antibody|FLK1 antibody|Kdr antibody|Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody|Kinase insert domain receptor antibody|KRD1 antibody|Ly73 antibody|Protein tyrosine kinase receptor FLK1 antibody|Protein-tyrosine kinase receptor flk-1 antibody| Tyrosine kinase growth factor receptor antibody|Vascular endothelial growth factor receptor 2 antibody|VEGFR 2 antibody|VEGFR antibody|VEGFR-2 antibody|VEGFR2 antibody|VGFR2_HUMAN antibody
Gene ID 3791
UniProt P35968
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 antibody (ABIN3043274) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for KDR is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 antibody (ABIN3043274) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-VEGFR2 antibody (ABIN3043274)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Product cited in:

Liu, Yang, Zhang, Shui, Song, Yao, Dai, Sun: "Fructopyrano-(1?4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner." in: International journal of clinical and experimental medicine, Vol. 7, Issue 11, pp. 3859-69, 2014

Wang, Liu, Wang, Wang: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." in: Oncology letters, Vol. 6, Issue 1, pp. 175-180, 2013

Sun, Chen, Song, Ren, Wei, Liu, Zhang, Lv: "Effect of visfatin on the function of endothelial progenitor cells in high-fat-fed obese rats and investigation of its mechanism of action." in: International journal of molecular medicine, Vol. 30, Issue 3, pp. 622-8, 2012

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN3043274) back to top
Supplier Images
Western Blotting (WB) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 29-60), (N-Term) antibody (ABIN3043274) anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 29-60), (N-Term) antibody