Mouse anti-Human IgG3 Antibody

Catalog No. ABIN3210023

Product Details

Target: IgG3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: It has been selected for its ability to recognize IgG3 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Native Protein IgG3 from Human Serum　There are four Immunoglobulin G subclasses, each numbered for their relative abundance in the body. These molecules are an important part of the immune system. All Immunoglobulin G molecules are made up of four chains of amino acids, connected at a central hinge in such a way that three ends extend from the center. The hinge within the Immunoglobulin G3 antibody is by far the longest in all the Immunoglobulin G subclasses. The length of the hinge allows Immunoglobulin G3 to quickly and effectively bind to and eliminate antigens. On the other hand, the length of the hinge also makes this subclass of Immunoglobulin G the most susceptible to damage from other molecules.
The antibody is a mouse monoclonal antibody raised against IgG3.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 µg/mL

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody

Background: There are four immunoglobulin G subclasses, each numbered for their relative abundance in the body. These molecules are an important part of the immune system. All immunoglobulin G molecules are made up of four chains of amino acids, connected at a central hinge in such a way that three ends extend from the center. The hinge within the immunoglobulin G3 antibody is by far the longest in all the immunoglobulin G subclasses. The length of the hinge allows immunoglobulin G3 to quickly and effectively bind to and eliminate antigens. On the other hand, the length of the hinge also makes this subclass of immunoglobulin G the most susceptible to damage from other molecules.