We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) Antibodies|
|Epitope||AA 900-950 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus), Human Alternatives|
|Conjugate||This GRIN1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Western Blotting (WB)
Product Details anti-GRIN1 AntibodyTarget Details GRIN1 Application Details Handling Images
|Specificity||NMDAR1 Antibody detects endogenous levels of mouse NMDAR1. It is predicted to react with human and rat NMDAR1 protein according to sequence homology.Positive Control: Mouse brain|
|Cross-Reactivity (Details)||NMDAR1 Antibody detects endogenous levels of mouse NMDAR1. It is predicted to react with human and rat NMDAR1 protein according to sequence homology.Positive Control: Mouse brain|
|Immunogen||KLH-coupled synthetic peptide within AA 900-950 of human NMDAR1|
|Plasmids, Primers & others|
Target Details GRIN1Product Details anti-GRIN1 Antibody Application Details Handling Images back to top
|Alternative Name||NMDAR1 (GRIN1 Antibody Abstract)|
N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NMDAR1 and one NR2A-D subunit. NMDAR1 subunits and NR2 subunits bind to the co-agonist glycine and the neurotransmitter glutamate, respectively.'PKC'an phosphorylate NMDAR1 of the receptor at Ser890/Ser896, and PKA can phosphorylate NMDAR1 at Ser897. The phosphorylation of NMDAR1 by'PKC'ecreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR. The phosphorylation of NMDAR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor.
NMDAR1 Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 900-950 of human NMDAR1 (Swiss Prot: Q05586).
|Pathways||Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity|
Application DetailsProduct Details anti-GRIN1 Antibody Target Details GRIN1 Handling Images back to top
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRIN1 Antibody Target Details GRIN1 Application Details Images back to top
|Buffer||PBS, pH 7.4, containing 0.02 % sodium azide.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Storage||4 °C/-20 °C|
|Storage Comment||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
ImagesProduct Details anti-GRIN1 Antibody Target Details GRIN1 Application Details Handling back to top