anti-Cow (Bovine) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Cow (Bovine)
1272
584
237
71
61
41
36
30
28
26
25
24
22
21
18
18
15
15
13
12
10
9
9
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
1347
525
303
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to PE
253
222
158
133
44
41
39
32
32
32
29
25
24
21
16
16
16
15
15
15
15
15
15
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1658
545
462
299
287
275
217
165
94
73
54
39
31
26
21
19
18
14
14
11
11
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2480170
$ 581.06
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.363859 ABIN182045 FACS FITC Mouse IgG2a Log in to see CC8 0
5.5 ABIN2480166 FACS IHC (fro) IP Mouse IgG2a Log in to see CC8 4
5.5 ABIN2480169 FACS FITC Mouse IgG2a Log in to see CC8 4
5.5 ABIN2480167 IHC (fro) FACS IHC (p) IP Mouse IgG1 Log in to see CC30 4
5.5 ABIN2480168 FACS Alexa Fluor 647 Mouse IgG2a Log in to see CC8 4
1 ABIN600636 FACS IHC IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN182047 FACS PE Mouse IgG2a Log in to see CC8 0
1 ABIN306129 FACS PE Mouse IgG2a Log in to see 0
1 ABIN206276 FACS FITC Mouse IgG2a Log in to see CC8 0
1 ABIN2226080 FACS IP IHC Mouse IgG1 Log in to see CC30 0
1 ABIN2225949 FACS IP IHC Mouse IgG2a Log in to see 8H2 0
1 ABIN2225942 FACS FITC Mouse IgG2a Log in to see 8H2 0
1 ABIN2225940 FACS IP IHC Mouse IgG2a Log in to see 8H2 0
1 ABIN2225941 FACS PE Mouse IgG2a Log in to see 8H2 0
1 ABIN1504728 IHC (fro) FACS IHC (p) IP Mouse IgG1 Log in to see CC30 0
1 ABIN641707 FACS PE Mouse Log in to see 0
1 ABIN641706 FACS FITC Mouse Log in to see 0

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Cow (Bovine)
(1272), (584), (237), (71), (61), (41), (36), (30), (28), (26), (25), (24), (22), (21), (18), (18), (15), (15), (13), (12), (10), (9), (9), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(1347), (525), (303), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to PE
(253), (222), (158), (133), (44), (41), (39), (32), (32), (32), (29), (25), (24), (21), (16), (16), (16), (15), (15), (15), (15), (15), (15), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1658), (545), (462), (299), (287), (275), (217), (165), (94), (73), (54), (39), (31), (26), (21), (19), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN2480170) Images
Characteristics Mouse anti Bovine CD4 antibody, clone CC8 recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskra et al. 1991).
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Bovine lymphocytes
Clone CC8
Isotype IgG2a
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN2480170) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
UniProt A7YY52
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN2480170) Images back to top
Application Notes Flow: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN2480170) Images back to top
Format Lyophilized
Reconstitution Reconstitute with 1 ml distilled water
Buffer Phosphate buffered saline, 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin, 5% Sucrose
Handling Advice DO NOT FREEZE This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage Comment Store at +4°C. This product should be stored undiluted.

References for anti-CD4 antibody (ABIN2480170)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Endsley, Furrer, Endsley, McIntosh, Maue, Waters, Lee, Estes: "Characterization of bovine homologues of granulysin and NK-lysin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2607-14, 2004

Background publications

Eskra, OReilly, Splitter: "Effect of monoclonal antibodies on in vitro function of T-cell subsets." in: Veterinary immunology and immunopathology, Vol. 27, Issue 1-3, pp. 215-22, 1991

Howard, Morrison, Bensaid, Davis, Eskra, Gerdes, Hadam, Hurley, Leibold, Letesson: "Summary of workshop findings for leukocyte antigens of cattle." in: Veterinary immunology and immunopathology, Vol. 27, Issue 1-3, pp. 21-7, 1991

Bensaid, Hadam: "Individual antigens of cattle. Bovine CD4 (BoCD4)." in: Veterinary immunology and immunopathology, Vol. 27, Issue 1-3, pp. 51-4, 1991

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN2480170) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (PE) (ABIN2480170) anti-CD4 Molecule (CD4) antibody (PE)