anti-Monkey CD4 antibody for ELISA

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Epitope
AA 270-320, N-Term
68
56
29
21
16
15
15
15
13
11
9
9
8
8
7
6
6
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine)
1337
636
241
78
69
45
35
30
28
27
26
25
25
23
23
19
18
17
16
15
13
12
11
9
9
7
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
1453
575
309
13
4
2
1
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
265
230
170
142
49
48
46
39
34
33
33
30
28
24
22
22
20
19
19
19
19
16
15
13
13
13
11
11
10
9
9
9
8
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
1805
581
485
355
314
300
220
174
99
80
54
39
32
28
23
21
18
14
14
11
11
9
6
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2747750
$ 462.69
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2749630 IP ELISA WB FITC Rabbit IgG AA 270-320, N-Term Log in to see Polyclonal 0
1 ABIN2749631 ELISA IHC IP WB FITC Rabbit IgG AA 408-458, C-Term Log in to see Polyclonal 0
1 ABIN2749629 ELISA IHC IP WB Rabbit IgG AA 408-458, C-Term Log in to see Polyclonal 0
1 ABIN5558707 ELISA IHC IP WB Biotin Rabbit C-Term Log in to see Polyclonal 0
1 ABIN5558706 ELISA IP WB Biotin Rabbit N-Term Log in to see Polyclonal 0

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope AA 270-320, N-Term
(68), (56), (29), (21), (16), (15), (15), (15), (13), (11), (9), (9), (8), (8), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine)
(1337), (636), (241), (78), (69), (45), (35), (30), (28), (27), (26), (25), (25), (23), (23), (19), (18), (17), (16), (15), (13), (12), (11), (9), (9), (7), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1453), (575), (309), (13), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(265), (230), (170), (142), (49), (48), (46), (39), (34), (33), (33), (30), (28), (24), (22), (22), (20), (19), (19), (19), (19), (16), (15), (13), (13), (13), (11), (11), (10), (9), (9), (9), (8), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(1805), (581), (485), (355), (314), (300), (220), (174), (99), (80), (54), (39), (32), (28), (23), (21), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2749632
Specificity The antibody labels a strong band of CD4 at 55 kDa in ABIN1741637 samples & in other cancer cell lines.
The antibody also labels a band of 28 kDa, the identity ofthis band is not fully understood but may represent a proteolytic degradation product of CD4 in SKNMC cells.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Monkey
Cross-Reactivity (Details) Does not cross react to any other cellular protein.
Characteristics CD4 selective antibodies were generated against a peptide taken from the human protein. The CD4 selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The CD4-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides CD4 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for CD4 antibody is also available, please inquire before ordering.
Purification Affinity Purified
Immunogen Synthetic peptide taken within amino acid region 270-320 on human CD4 protein.
Isotype IgG
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Human T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single chain trans membranous glycoprotein (55 kDa) which belongs to the immunoglobulin superfamily. CD4 (Cluster of Differentiation 4) consists of a cytoplasmic tail, four immunoglobulin-like external domains (D1-D4), and a transmembrane domain. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and at a lower level on monocytes, regulatory T cells, tissue macrophages, granulocytes, Langerhans cells and dendritic cells. It is also expressed in specific regions of the brain. It is an important component of the immune system and is the cellular receptor for HIV-1. This protein initiates the early phase of T-cell activation, and is an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. CD4 plays a role in the regulation of T-B lymphocyte adhesion in the absence of antigen recognition, and in thymic differentiation. Subpopulations of suppressor or cytotoxic T cells are identified among CD4-positive T cells. The intracellular domain of the antigen is associated with p56 lck protein tyrosine kinase. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. The antigen interacts directly with major histocompatibility complex (MHC) class II molecules on the surface of the antigen presenting cell via its extracellular domain. It is also a major receptor for the human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors thus making the CD4 count an important factor to decide when to begin treatment for HIV-infected patients.The human CD4-specific gene maps to chromosome 12pter-p12.
NCBI Accession NP_000607
UniProt P01730
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
Format Liquid
Concentration 0.55-0.75 μg/μL
Handling Advice Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.
Storage -20 °C
Storage Comment For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.