anti-Mouse (Murine) CD4 antibody for Depletion

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1306
640
248
78
68
44
34
29
26
26
26
24
23
23
21
19
18
16
16
15
12
10
9
9
7
6
5
5
4
3
3
3
2
2
2
2
1
1
1
1
Host
Rat
1439
578
308
11
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to Biotin
267
233
169
143
49
48
46
39
36
33
30
30
28
24
22
22
20
19
19
19
19
16
15
13
13
13
11
11
10
9
9
9
8
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Blocking Reagent (BR), Depletion (Dep), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
1822
581
473
358
321
304
214
172
96
78
54
39
31
28
23
21
18
14
14
11
11
9
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2661074
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2661073 BR Dep FACS IP IHC Biotin Rat IgG2b kappa Log in to see GK1-5 11

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1306), (640), (248), (78), (68), (44), (34), (29), (26), (26), (26), (24), (23), (23), (21), (19), (18), (16), (16), (15), (12), (10), (9), (9), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1439), (578), (308), (11), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to Biotin
(267), (233), (169), (143), (49), (48), (46), (39), (36), (33), (30), (30), (28), (24), (22), (22), (20), (19), (19), (19), (19), (16), (15), (13), (13), (13), (11), (11), (10), (9), (9), (9), (8), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Depletion (Dep), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(1822), (581), (473), (358), (321), (304), (214), (172), (96), (78), (54), (39), (31), (28), (23), (21), (18), (14), (14), (11), (11), (9), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN2661074) Images
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone GK1-5
Isotype IgG2b kappa
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN2661074) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN2661074) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN2661074) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD4 antibody (ABIN2661074)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Gavett, Chen, Finkelman, Wills-Karp: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, 1994

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Ghobrial, Boublik, Winn, Auchincloss: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, 1989

Israelski, Araujo, Conley, Suzuki, Sharma, Remington: "Treatment with anti-L3T4 (CD4) monoclonal antibody reduces the inflammatory response in toxoplasmic encephalitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 3, pp. 954-8, 1989

Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, 1983

Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (Biotin) (ABIN2661074) anti-CD4 Molecule (CD4) antibody (Biotin)