anti-Mouse (Murine) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Epitope
Extracellular
69
53
30
20
16
15
15
15
13
9
8
8
6
6
6
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
1262
576
228
69
61
41
34
30
28
26
25
25
22
21
18
18
15
13
11
10
10
9
8
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rat
1324
516
306
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
247
216
153
127
41
40
38
32
30
29
29
25
24
20
16
15
15
15
15
15
15
14
14
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
1648
531
461
302
281
267
214
167
90
72
49
39
31
26
21
19
18
14
13
11
11
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN343704
$ 257.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
22.075203 ABIN457307 FACS Func ICC IHC (f) IP APC Rat IgG2b Extracellular Log in to see GK1-5 9
22.075203 ABIN509566 FACS Func ICC IHC (f) IP FITC Rat IgG2b Extracellular Log in to see GK1-5 9
22.075203 ABIN457367 FACS Func ICC IHC (f) IP PE Rat IgG2b Extracellular Log in to see GK1-5 9
22.075203 ABIN2749027 Func IHC (f) ICC FACS IP Rat IgG2b Log in to see GK1-5 9
22.075203 ABIN2749008 Func IHC (f) ICC FACS IP Rat IgG2b Log in to see GK1-5 9
21.579416 ABIN2145315 FACS APC Rat IgG2b Log in to see 2
21.579416 ABIN2145317 FACS APC Rat IgG2b Log in to see 2
21.579416 ABIN2145323 FACS PE-Cy5 Rat IgG2b Log in to see 2
21.579416 ABIN2145325 FACS PE-Cy5 Rat IgG2b Log in to see 2
21.579416 ABIN2145431 FACS PE Rat IgG2b Log in to see 2
21.579416 ABIN2145433 FACS PE Rat IgG2b Log in to see 2
20.575203 ABIN135103 FACS PE Rat IgG2b Log in to see GK1-5 3
16.075203 ABIN135104 FACS SPRD Rat IgG2b Log in to see GK1-5 2
16.075203 ABIN135101 FACS FITC Rat IgG2b Log in to see GK1-5 2
16.075203 ABIN135102 FACS Biotin Rat IgG2b Log in to see GK1-5 2
16.075203 ABIN135100 FACS FITC Rat IgG2b Log in to see GK1-5 2
16.075203 ABIN135099 FACS Rat IgG2b Log in to see GK1-5 2
16.075203 ABIN135105 FACS Rat IgG2b Log in to see GK1-5 2
16.075203 ABIN376484 FACS PE Rat IgG2b Log in to see GK1-5 0
16.075203 ABIN375841 FACS APC-Cy5.5 Rat IgG2b Log in to see GK1-5 0

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Extracellular
(69), (53), (30), (20), (16), (15), (15), (15), (13), (9), (8), (8), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(1262), (576), (228), (69), (61), (41), (34), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (13), (11), (10), (10), (9), (8), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rat
(1324), (516), (306), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(247), (216), (153), (127), (41), (40), (38), (32), (30), (29), (29), (25), (24), (20), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(1648), (531), (461), (302), (281), (267), (214), (167), (90), (72), (49), (39), (31), (26), (21), (19), (18), (14), (13), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN343704) Images
Specificity The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Immunogen Mouse CTL clone V4 cells
Clone GK1-5
Isotype IgG2b
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN343704) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN343704) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN343704) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD4 antibody (ABIN343704)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Hu, Watson, Zhang, Graf, Wang, Sartor, Howden, Fletcher, Alexander: "Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6593-603, 2008

Yi, Zhen, Zeng, Zhang, Zhao: "Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice." in: Transplantation, Vol. 85, Issue 8, pp. 1167-74, 2008

Felix, Donermeyer, Horvath, Walters, Gross, Suri, Allen: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, 2007

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, 1997

Gavett, Chen, Finkelman, Wills-Karp: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, 1994

Godfrey, Kennedy, Gately, Hakimi, Hubbard, Zlotnik: "IL-12 influences intrathymic T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2729-35, 1994

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992

Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, 1983

Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983

Background publications

Wilde, Marrack, Kappler, Dialynas, Fitch et al.: "Evidence implicating L3T4 in class II MHC antigen reactivity, monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2178-83, 1983