anti-Mouse (Murine) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1234
638
248
81
67
44
36
29
26
26
22
21
19
18
16
15
12
10
10
9
8
7
6
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rat
1378
581
293
12
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
168
138
50
50
48
41
35
34
30
29
26
22
22
22
20
19
19
19
19
16
15
15
13
13
13
11
9
9
8
6
5
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1748
510
466
358
330
305
171
170
101
78
39
38
32
31
30
25
18
14
14
11
11
9
6
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN118115
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.98401 ABIN114143 FACS APC Rat IgG2a Log in to see CT-CD4 10
13.98401 ABIN2657424 FACS ICFC Alexa Fluor 488 Rat IgG2b kappa Log in to see GK1-5 13
13.98401 ABIN255884 FACS IHC (fro) IHC Rat IgG2b Log in to see YTS191-1 0
13.98401 ABIN4292794 FACS ICC IF IHC IHC (fro) Rat IgG kappa Log in to see GK1-5 3
13.98401 ABIN114148 FACS FITC Rat IgG2a Log in to see CT-CD4 7
13.98401 ABIN114145 FACS Biotin Rat IgG2a Log in to see CT-CD4 6
13.98401 ABIN118164 FACS FITC Rat IgG2a Non-Polymorphic Site Log in to see YTS177-9 4
13.98401 ABIN4898369 FACS Fluorescein Rat IgG2b Log in to see GK1-5 3
13.98401 ABIN4898370 FACS Fluorescein Rat IgG2b Log in to see GK1-5 3
13.98401 ABIN4898375 FACS PE Rat IgG2b Log in to see GK1-5 3
13.98401 ABIN4898376 FACS PE Rat IgG2b Log in to see GK1-5 3
13.98401 ABIN114151 FACS PE Rat IgG2a Log in to see CT-CD4 7
13.98401 ABIN114150 FACS PE Rat IgG2a Log in to see CT-CD4 7
13.98401 ABIN4292797 FACS FITC Rat IgG kappa Log in to see GK1-5 1
13.98401 ABIN2665991 FACS IF IHC Rat IgG2b kappa Log in to see GK1-5 9
13.98401 ABIN2663972 FACS PE Rat IgG2b kappa Log in to see GK1-5 9
13.98401 ABIN2659912 BR FACS PerCP Rat IgG2b kappa Log in to see GK1-5 9
13.98401 ABIN2661074 BR Dep FACS IP IHC Biotin Rat IgG2b kappa Log in to see GK1-5 9
13.98401 ABIN2662124 FACS Pacific Blue Rat IgG2b kappa Log in to see GK1-5 9
13.98401 ABIN2662047 FACS FITC Rat IgG2b kappa Log in to see GK1-5 9

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1234), (638), (248), (81), (67), (44), (36), (29), (26), (26), (22), (21), (19), (18), (16), (15), (12), (10), (10), (9), (8), (7), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1378), (581), (293), (12), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(266), (227), (168), (138), (50), (50), (48), (41), (35), (34), (30), (29), (26), (22), (22), (22), (20), (19), (19), (19), (19), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(1748), (510), (466), (358), (330), (305), (171), (170), (101), (78), (39), (38), (32), (31), (30), (25), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN118115) Images
Specificity This antibody recognises the CD4 cell surface antigen, expressed by a subset of T-lymphocytes. This clone exhibits depleting activity when used in vivo (Azide Free CD4 Antibody Cat.-No SM005LE is recommended for this purpose).
Purification Affinity Chromatography on Protein G
Clone YTS191-1
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN118115) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 12504
NCBI Accession NP_038516
UniProt P06332
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN118115) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 106 cells.
Immunohistochemistry on Frozen Sections: The epitope recognised by this antibody is reported to be sensitive to Formaldehyde Fixation and tissue processing. The use of Acetone fixation for frozen sections is recommended.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN118115) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 antibody (ABIN118115)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Taskin, Sasso, Dimaki, Svendsen, Castillo-León: "Combined cell culture-biosensing platform using vertically aligned patterned peptide nanofibers for cellular studies." in: ACS applied materials & interfaces, Vol. 5, Issue 8, pp. 3323-8, 2013

Abdulreda, Faleo, Molano, Lopez-Cabezas, Molina, Tan, Echeverria, Zahr-Akrawi, Rodriguez-Diaz, Edlund, Leibiger, Bayer, Perez, Ricordi, Caicedo, Pileggi, Berggren: "High-resolution, noninvasive longitudinal live imaging of immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. 12863-8, 2011

Nakashima, Terabe, Berzofsky, Husain, Puri: "A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 4935-46, 2011

Scotland, Stables, Madalli, Watson, Gilroy: "Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice." in: Blood, Vol. 118, Issue 22, pp. 5918-27, 2011

Eller, Wolf, Huber, Metz, Mayer, McKenzie, Maurer, Rosenkranz, Wolf: "IL-9 production by regulatory T cells recruits mast cells that are essential for regulatory T cell-induced immune suppression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 1, pp. 83-91, 2010

Grimm, Gasser, Bueter, Strehl, Wang, Nichiporuk, Meyer, Germer, Waaga-Gasser, Thalheimer: "Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases." in: BMC cancer, Vol. 10, pp. 82, 2010

Huber, Tagwerker, Heininger, Mayer, Rosenkranz, Eller: "The proteasome inhibitor bortezomib aggravates renal ischemia-reperfusion injury." in: American journal of physiology. Renal physiology, Vol. 297, Issue 2, pp. F451-60, 2009

Gaupp, Cannella, Raine: "Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines." in: The American journal of pathology, Vol. 173, Issue 1, pp. 119-29, 2008

Jégou, Chan, Schouft, Griffiths, Neal, Gasque, Vaudry, Fontaine: "C3d binding to the myelin oligodendrocyte glycoprotein results in an exacerbated experimental autoimmune encephalomyelitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 5, pp. 3323-31, 2007

Wolf, Hochegger, Wolf, Rumpold, Gastl, Tilg, Mayer, Gunsilius, Rosenkranz: "CD4+CD25+ regulatory T cells inhibit experimental anti-glomerular basement membrane glomerulonephritis in mice." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 5, pp. 1360-70, 2005

Gardinier, Khoury, Lehn: "Enforced helicity: efficient access to self-organized helical molecular strands by the imine route." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 6, Issue 22, pp. 4124-31, 2000

Higgins, McDonald, Whittle, Crockett, Shields, MacDonald et al.: "Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 1, pp. 486-93, 1999

Bemelman, Honey, Adams, Cobbold, Waldmann: "Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 6, pp. 2645-8, 1998

Bourmeyster, Stasia, Garin, Gagnon, Boquet, Vignais: "Copurification of rho protein and the rho-GDP dissociation inhibitor from bovine neutrophil cytosol. Effect of phosphoinositides on rho ADP-ribosylation by the C3 exoenzyme of Clostridium botulinum." in: Biochemistry, Vol. 31, Issue 51, pp. 12863-9, 1993

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN118115) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (ABIN118115) Staining of mouse spleen cells with Rat anti Mouse CD4 (ABIN118115)