anti-Mouse (Murine) CD4 antibody for Immunohistochemistry (Zinc-fixed Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1235
638
248
81
67
44
36
29
26
26
22
21
19
18
16
15
12
10
10
9
8
7
6
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rat
1379
581
293
12
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
168
138
50
50
48
41
35
34
30
29
26
22
22
22
20
19
19
19
19
16
15
15
13
13
13
11
9
9
8
6
5
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
1748
510
466
358
330
304
171
170
101
78
39
38
31
31
30
25
18
14
13
11
11
9
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN2689225
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689224 IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see RM4 13
1 ABIN2689231 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 9
1 ABIN2689230 IHC (f) IHC (fro) FACS IHC (zinc) Rat IgG2a, kappa Log in to see RM4 6
1 ABIN1176846 BR CyTox IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 14

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1235), (638), (248), (81), (67), (44), (36), (29), (26), (26), (22), (21), (19), (18), (16), (15), (12), (10), (10), (9), (8), (7), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1379), (581), (293), (12), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(266), (227), (168), (138), (50), (50), (48), (41), (35), (34), (30), (29), (26), (22), (22), (22), (20), (19), (19), (19), (19), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
(1748), (510), (466), (358), (330), (304), (171), (170), (101), (78), (39), (38), (31), (31), (30), (25), (18), (14), (13), (11), (11), (9), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN2689225) Images
Brand BD Pharmingen™
Characteristics The H129.19 antibody reacts with the CD4 (L3T4) differentiation antigen expressed on thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. CD4 has also been detected on pluirpotent hematopoietic stem cells, bone marrow myeloid precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 is expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine lck. H129.19 mAb blocks binding of the anti-mouse CD4 mAbs Gk1.5 (Cat. No. 557307/553729) and RM4-5 (Cat. No. 553046/553047), but not RM4-4 (Cat. No. 553055) antibody. mAb H129.19 inhibits various responses of T helper cells to antigenic or mitogenic stimuli. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone H129.19 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen A.TH mouse CTL clone A15.1.17
Clone H129-19
Isotype IgG2a, kappa

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN2689225) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Synonyms: L3T4
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN2689225) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN2689225) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD4 antibody (ABIN2689225)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Martín, del Hoyo, Anjuère, Ruiz, Arias, Marín, Ardavín: "Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors." in: Blood, Vol. 96, Issue 7, pp. 2511-9, 2000

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, 1995

Guo, Watanabe, Mori, Mori: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, 1995

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Wineman, Gilmore, Gritzmacher, Torbett, Müller-Sieburg: "CD4 is expressed on murine pluripotent hematopoietic stem cells." in: Blood, Vol. 80, Issue 7, pp. 1717-24, 1992

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992

Wu, Scollay, Egerton, Pearse, Spangrude, Shortman: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, 1991

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, 1989

Ghobrial, Boublik, Winn, Auchincloss: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, 1989

Regnier-Vigouroux, Blanc, Pont, Marchetto, Pierres: "Accessory molecules and T cell activation. I. Antigen receptor avidity differentially influences T cell sensitivity to inhibition by monoclonal antibodies to LFA-1 and L3T4." in: European journal of immunology, Vol. 16, Issue 11, pp. 1385-90, 1987

Pont, Régnier-Vigouroux, Marchetto, Pierres: "Accessory molecules and T cell activation. II. Antibody binding to L3T4a inhibits Ia-independent mouse T cell proliferation." in: European journal of immunology, Vol. 17, Issue 3, pp. 429-32, 1987

Naquet, Malissen, Bekkhoucha, Pont, Pierres, Hood, Pierres: "L3T4 but not LFA-1 participates in antigen presentation by Ak-positive L-cell transformants." in: Immunogenetics, Vol. 22, Issue 3, pp. 247-56, 1985

Pierres, Naquet, Van Agthoven, Bekkhoucha, Denizot, Mishal, Schmitt-Verhulst, Pierres et al.: "A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 6, pp. 2775-82, 1984