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|Antigen||CD4 Molecule (CD4) Antibodies|
|Epitope||C-Term, AA 400-458 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
|12 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN439030) Images
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an C-terminal region of the human CD4 protein (within residues 400-458). [Swiss-Prot P01730]|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN439030) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||Gene Symbol: CD4|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN439030) Images back to top
|Application Notes||Western Blot 1 μg/mL, Simple Western 1:100, Immunohistochemistry 1:50-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:500This CD4 antibody is useful for ICC, Western Blot, and IHC-paraffin embedded sections. In WB, a band is seen ~45 kDa. In ICC/IF, membrane staining was observed in human brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Jurkat cells. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western Blot protocol specific for CD4 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 25 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Protocol Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN439030) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-CD4 antibody (ABIN439030)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
|Product cited in:||
Lin, Wang, Wang, Ling: "Quercetin protects against atherosclerosis by inhibiting dendritic cell activation." in: Molecular nutrition & food research, Vol. 61, Issue 9, 2017 (Sample species: Mouse (Murine)).
Song, Endo, Song, Shibata, Koji: "5-aza-2'-deoxycytidine impairs mouse spermatogenesis at multiple stages through different usage of DNA methyltransferases." in: Toxicology, Vol. 361-362, pp. 62-72, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence
Ren, Li, Tian, Nie, Wang, Li, Zheng: "Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice." in: Scientific reports, Vol. 5, pp. 15730, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Gervais, Desantis, Pagendarm, Nelson, Enger, Skarstein, Liaaen Jensen, Larsen: "Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 298, Issue 9, pp. 1622-34, 2015 Method employed by authors: Chromatin Immunoprecipitation (ChIP)
Yang, Vethanayagam, Dong, Cai, Hu: "Activation of the antigen presentation function of mononuclear phagocyte populations associated with the basilar membrane of the cochlea after acoustic overstimulation." in: Neuroscience, Vol. 303, pp. 1-15, 2015 Method employed by authors: Flow Cytometry (FACS)
Tabibian, OHara, Trussoni, Tietz, Splinter, Mounajjed, Hagey, LaRusso: "Absence of the intestinal microbiota exacerbates hepatobiliary disease in a murine model of primary sclerosing cholangitis." in: Hepatology (Baltimore, Md.), Vol. 63, Issue 1, pp. 185-96, 2015 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).
Migita, Sho, Shimada, Yasuda, Yamato, Takayama, Matsumoto, Wakatsuki, Hotta, Tanaka, Ito, Konishi, Nakajima: "Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma." in: Cancer, Vol. 120, Issue 6, pp. 808-17, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
Helsby, Leader, Fenn, Gulsen, Bryant, Doughton, Sharpe, Whitley, Caunt, James, Pope, Kelly, Chalmers: "CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited." in: BMC cell biology, Vol. 15, pp. 6, 2014 (Sample species: Human).
Yasuda, Sho, Yamato, Yoshiji, Wakatsuki, Nishiwada, Yagita, Nakajima: "Simultaneous blockade of programmed death 1 and vascular endothelial growth factor receptor 2 (VEGFR2) induces synergistic anti-tumour effect in vivo." in: Clinical and experimental immunology, Vol. 172, Issue 3, pp. 500-6, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Zheng, Li, Tian, Ren, Du, Li: "Notch γ-secretase inhibitor dibenzazepine attenuates angiotensin II-induced abdominal aortic aneurysm in ApoE knockout mice by multiple mechanisms." in: PLoS ONE, Vol. 8, Issue 12, pp. e83310, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Hwang, Ahn, Park, Ha, Song, Jee: "An acidic polysaccharide of Panax ginseng ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells." in: Immunology letters, Vol. 138, Issue 2, pp. 169-78, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
Lu, Li, Wang, Zheng, Pu, Ding, Li, Xu, Gao, Chen: "The effect of programmed cryopreservation on immunogenicity of bladder mucosa in New Zealand rabbits." in: Cryobiology, Vol. 64, Issue 1, pp. 27-32, 2011 (Sample species: Rabbit). Further details: Immunohistochemistry
ImagesProduct Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN439030) back to top