anti-Rat (Rattus) CD4 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Rat (Rattus)
1262
577
227
69
61
41
34
30
28
26
25
25
22
21
18
18
15
13
11
10
10
9
8
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
1323
517
306
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
247
216
153
127
41
40
38
32
30
29
29
25
24
20
16
15
15
15
15
15
15
14
14
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
1648
531
461
301
281
268
214
167
91
73
49
39
31
26
21
19
18
14
13
11
11
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2689233
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN2689232 IHC (f) IHC (fro) FACS Mouse IgG2a, kappa Log in to see OX 6

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Rat (Rattus)
(1262), (577), (227), (69), (61), (41), (34), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (13), (11), (10), (10), (9), (8), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(1323), (517), (306), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(247), (216), (153), (127), (41), (40), (38), (32), (30), (29), (29), (25), (24), (20), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(1648), (531), (461), (301), (281), (268), (214), (167), (91), (73), (49), (39), (31), (26), (21), (19), (18), (14), (13), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN2689233) Images
Brand BD Pharmingen™
Characteristics The OX-38 antibody has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-38 antibody has been reported to bind to the same epitope of CD4 as that recognized by W3/25 mAb, which is a different epitope than that recognized by OX-35 mAb (Cat. No. 554837). In vivo blocking of some cell-mediated immune responses by mAb OX-38 has been reported. Injection of OX-38 mAb induces allograft unresponsiveness in rats, with varying results depending on the rat strain used (high or low responder). Furthermore, in vivo depletion of CD4+ lymphocytes has been reported with this antibody. Immunohistochemical staining of rat CD4 + T lymphocytes. Frozen sections of normal rat spleen were stained with Purified Mouse Anti-Rat CD4 antibody (Cat. No. 550297). CD4+ T lymphocytes can be identified by the intense brown labeling of their cell surface membranes. Amplification 20X.

BD Pharmingen™ Purified Mouse Anti-Rat CD4 - Purified - Clone OX-38 - Isotype Mouse IgG2a, κ - Reactivity Rat - 1 mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat thymocyte glycoproteins
Clone OX
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN2689233) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN2689233) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN2689233) Images back to top
Format Liquid
Concentration 125 μg/mL
Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD4 antibody (ABIN2689233)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Liu, Zhang, Jenkins, MacPherson: "Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, 1998

Yin, Fathman: "Tissue-specific effects of anti-CD4 therapy in induction of allograft unresponsiveness in high and low responder rats." in: Transplant immunology, Vol. 3, Issue 3, pp. 258-64, 1996

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, 1993

Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." in: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, 1992

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, 1985