anti-Human CD80 antibody for Immunoprecipitation

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Epitope
Extracellular Domain
26
15
15
15
14
12
11
11
10
8
7
6
6
5
5
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
452
306
47
11
10
6
3
2
2
2
1
1
Host
Mouse
393
127
98
91
63
16
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
74
72
53
43
19
17
15
13
8
8
7
7
6
6
6
6
6
6
6
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
529
214
173
152
114
94
87
38
35
26
22
21
12
9
8
6
6
6
5
5
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN94239
$ 360.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.026281 ABIN94240 FACS IP FITC Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
24.026281 ABIN192195 FACS IP PE Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
24.026281 ABIN94241 FACS IP APC Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
24.026281 ABIN457391 FACS IP PerCP Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
16.452717 ABIN514174 IP ELISA WB Mouse IgM kappa AA 1-288 Log in to see 3E10 0
16.452717 ABIN514175 IP ELISA WB Mouse IgM kappa AA 1-288 Log in to see 4A4 0
13.452718 ABIN514173 IP Rabbit AA 1-288 Log in to see Polyclonal 0
12.026281 ABIN2137014 FACS IP IHC Mouse IgG1 kappa Log in to see 2D10-4 0
12.026281 ABIN1690586 IP Sepharose Rabbit Log in to see Polyclonal 0
12.026281 ABIN1690239 IP Magnetic Particles Rabbit Log in to see Polyclonal 0
8.5 ABIN2472591 FACS IP WB Mouse IgG1 Log in to see MEM-233 4
8.5 ABIN535467 FACS IP Mouse IgG1 Log in to see MEM-233 3
4 ABIN205436 FACS IHC IHC (fro) IP WB Mouse IgG1, kappa Log in to see 2D10 0
4 ABIN3037602 FACS IP PerCP Mouse IgG1 Extracellular Domain Log in to see 0
4 ABIN3037603 FACS IP PE Mouse IgG1 Extracellular Domain Log in to see 0
1 ABIN119435 FACS IP Mouse IgG1 Log in to see MEM-233 8
1 ABIN2565051 FACS IP WB FITC Mouse IgG1 Log in to see MEM-233 0
1 ABIN1495445 IP Sepharose Rabbit AA 57-71 Log in to see Polyclonal 0
1 ABIN1495444 IP Magnetic Particles Rabbit AA 57-71 Log in to see Polyclonal 0
1 ABIN1837728 FACS IP WB Mouse IgG1 Log in to see MEM-233 0

General

Antigen CD80 Antibodies
Epitope Extracellular Domain
(26), (15), (15), (15), (14), (12), (11), (11), (10), (8), (7), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(452), (306), (47), (11), (10), (6), (3), (2), (2), (2), (1), (1)
Host Mouse
(393), (127), (98), (91), (63), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(74), (72), (53), (43), (19), (17), (15), (13), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(529), (214), (173), (152), (114), (94), (87), (38), (35), (26), (22), (21), (12), (9), (8), (6), (6), (6), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN94239) Images
Specificity The antibody MEM-233 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Extracellular domain of human CD80 fused to human IgG1(Fc)
Clone MEM-233
Isotype IgG1

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 antibody (ABIN94239) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 antibody (ABIN94239) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 antibody (ABIN94239) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD80 antibody (ABIN94239)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Silk, Leishman, Nishimoto, Reddy, Fairchild: "Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 172420, 2012

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011

Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, 2007

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, 2007

Campioni, Moretti, Ferrari, Punturieri, Castoldi, Lanza: "Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment." in: Haematologica, Vol. 91, Issue 3, pp. 364-8, 2006

Tan, Beutelspacher, Wang, McClure, Ritter, Lombardi, George: "Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 11, Issue 5, pp. 790-800, 2005

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, 2003

Background publications

Yadav, Judkowski, Flodstrom-Tullberg, Sterling, Redmond, Sherman, Sarvetnick: "B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3631-9, 2004

Vasilevko, Ghochikyan, Holterman, Agadjanyan: "CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA." in: DNA and cell biology, Vol. 21, Issue 3, pp. 137-49, 2002

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN94239) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 (CD80) (Extracellular Domain) antibody (ABIN94239) Surface staining of RAJI human Burkitt lymphoma cell line with anti-human CD80 (MEM-2...