anti-Mouse (Murine) CD80 antibody for Western Blotting

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Epitope
AA 37-245
33
24
22
15
15
15
14
11
10
10
8
7
6
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
476
306
48
11
10
6
4
3
2
2
1
1
1
Host
Goat
422
125
109
100
43
16
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
78
77
57
43
20
20
18
17
12
10
10
8
8
8
8
7
7
7
6
6
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
557
210
173
152
122
93
74
45
37
28
26
17
8
8
6
6
6
5
5
4
2
2
2
2
1
1
1
Supplier
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Catalog No. ABIN4900307
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1803138 ABIN4903203 FACS IF IHC WB Mouse IgG Log in to see 0
1 ABIN678683 IF (p) IHC (p) WB Rabbit IgG AA 270-321 Log in to see Polyclonal 5
1 ABIN2661827 ELISA (Capture) WB FITC Hamster IgG Log in to see 16-10A1 11
1 ABIN2657475 ICC IF IP IHC ELISA WB Alexa Fluor 488 Hamster IgG Log in to see 16-10A1 11
1 ABIN2661826 ELISA (Capture) WB FITC Hamster IgG Log in to see 16-10A1 13
1 ABIN2657476 ICC IF IP IHC ELISA WB Alexa Fluor 488 Hamster IgG Log in to see 16-10A1 11
1 ABIN678692 IHC (p) WB HRP Rabbit IgG AA 270-321 Log in to see Polyclonal 0
1 ABIN5530457 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN678685 IHC (p) WB Biotin Rabbit IgG AA 270-321 Log in to see Polyclonal 0
1 ABIN465073 ELISA WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN465082 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN5674978 WB Biotin Rabbit IgG AA 1-100 Log in to see Polyclonal 0
1 ABIN5674979 WB HRP Rabbit IgG AA 1-100 Log in to see Polyclonal 0
1 ABIN2746298 ELISA IHC IP WB FITC Rabbit IgG AA 1-100 Log in to see Polyclonal 0
1 ABIN2746297 ELISA IHC IP WB Rabbit IgG AA 1-100 Log in to see Polyclonal 0
1 ABIN116383 EIA WB Rabbit Log in to see Polyclonal 0
1 ABIN116384 EIA WB Rabbit Log in to see Polyclonal 0
1 ABIN1854865 IF/ICC IHC WB Mouse IgG2b Extracellular Log in to see 0
1 ABIN5697128 ELISA FACS WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5674977 WB Rabbit IgG AA 1-100 Log in to see Polyclonal 0

General

Antigen CD80 Antibodies
Epitope AA 37-245
(33), (24), (22), (15), (15), (15), (14), (11), (10), (10), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(476), (306), (48), (11), (10), (6), (4), (3), (2), (2), (1), (1), (1)
Host Goat
(422), (125), (109), (100), (43), (16)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(78), (77), (57), (43), (20), (20), (18), (17), (12), (10), (10), (8), (8), (8), (8), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(557), (210), (173), (152), (122), (93), (74), (45), (37), (28), (26), (17), (8), (8), (6), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900307) Images
Specificity Detects mouse B7-1/CD80 in ELISAs and Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80, Asp37-Lys245, Accession # Q00609
Isotype IgG

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 antibody (ABIN4900307) Images back to top
Antigen
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Background Alternative Names: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 Molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt Q00609
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 antibody (ABIN4900307) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.15-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 antibody (ABIN4900307) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD80 antibody (ABIN4900307)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Garg, Delaney, Toubai, Ghosh, Reddy, Banerjee, Yung: "Aging is associated with increased regulatory T-cell function." in: Aging cell, Vol. 13, Issue 3, pp. 441-8, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Sofra, Mansour, Liu, Gao, Primpidou, Wang, Li: "Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection." in: European journal of immunology, Vol. 39, Issue 1, pp. 85-95, 2009 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

He, Li, Chen, Zhang, Chen, Hayashida, Zhu, Tseng: "Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract." in: Investigative ophthalmology & visual science, Vol. 49, Issue 10, pp. 4468-75, 2008 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900307) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes eith...
Western Blotting (WB) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mou...
Neutralization (Neut) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. R...
Immunocytochemistry (ICC) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) anti-CD80 (CD80) (AA 37-245) antibody (Image 4)