anti-Rat (Rattus) CD80 antibody for Western Blotting

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Epitope
AA 227-245, C-Term
33
24
19
15
15
15
13
11
10
10
8
7
6
5
5
5
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
498
294
48
11
11
6
3
3
3
2
1
1
Host
Rabbit
442
125
100
94
46
17
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
76
75
56
44
21
19
19
17
10
9
9
7
7
7
7
7
7
6
6
6
6
6
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
557
222
173
157
119
101
82
43
41
26
23
22
13
8
8
6
6
5
5
5
4
2
2
2
2
1
1
1
Options
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Catalog No. ABIN5518665
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0797327 ABIN2718314 FACS IF WB Mouse IgG1 Log in to see 1E9 0
3.0797327 ABIN3030426 WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN678683 IF (p) IHC (p) WB Rabbit IgG AA 270-321 Log in to see Polyclonal 5
1 ABIN678692 IHC (p) WB HRP Rabbit IgG AA 270-321 Log in to see Polyclonal 0
1 ABIN2618750 WB Rabbit IgG AA 227-245 Log in to see Polyclonal 0
1 ABIN678685 IHC (p) WB Biotin Rabbit IgG AA 270-321 Log in to see Polyclonal 0
1 ABIN2352197 WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2746298 ELISA IHC IP WB FITC Rabbit IgG AA 1-100 Log in to see Polyclonal 0
1 ABIN6246459 FACS ICC IF WB Mouse IgG1 Log in to see OTI1E9 0
1 ABIN2746297 ELISA IHC IP WB Rabbit IgG AA 1-100 Log in to see Polyclonal 0
1 ABIN5557640 ELISA IHC IP WB Biotin Rabbit Log in to see Polyclonal 0

General

Antigen CD80 Antibodies
Epitope AA 227-245, C-Term
(33), (24), (19), (15), (15), (15), (13), (11), (10), (10), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(498), (294), (48), (11), (11), (6), (3), (3), (3), (2), (1), (1)
Host Rabbit
(442), (125), (100), (94), (46), (17)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(76), (75), (56), (44), (21), (19), (19), (17), (10), (9), (9), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(557), (222), (173), (157), (119), (101), (82), (43), (41), (26), (23), (22), (13), (8), (8), (6), (6), (5), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.
Sequence DAHVSQNFTW EKPPEDPPD
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.
Gene Name: CD80 Molecule
Protein Name: CD80 antigen, isoform CRA_a
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.
Isotype IgG
Plasmids, Primers & others

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Synonyms: Activation B7 1 antigen antibody|Activation B7-1 antigen antibody|Activation B71 antigen antibody|B lymphocyte activation antigen B7 antibody|B7 antibody|B7-1 antibody|B7-1 antigen antibody|BB1 antibody|CD 80 antibody|CD28 antigen ligand 1 antibody|CD28 antigen ligand 1 B7 1 antigen antibody|CD28 antigen ligand 1 B71 antigen antibody|CD28LG antibody|CD28LG1 antibody|CD80 antibody|CD80 antigen antibody|CD80 antigen precursor antibody|CD80 Molecule antibody|CD80_HUMAN antibody|Costimulatory factor CD80 antibody|CTLA 4 counter receptor B7.1 antibody|CTLA-4 counter-receptor B7.1 antibody|LAB 7 antibody|LAB7 antibody|T lymphocyte activation antigen CD80 antibody|T-lymphocyte activation antigen CD80 antibody
UniProt G3V671
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, The detection limit for CD80 is approximately 1.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD80 (CD80) (AA 227-245), (C-Term) antibody (ABIN5518665) Anti- CD80 antibody, Western blottingAll lanes: Anti CD80 at 0.5ug/mlWB: Rat Thymus ...