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|Epitope||AA 227-245, C-Term Alternatives|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD80 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-CD80 AntibodyTarget Details CD80 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.
Gene Name: CD80 Molecule
Protein Name: CD80 antigen, isoform CRA_a
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.|
|Plasmids, Primers & others|
Target Details CD80Product Details anti-CD80 Antibody Application Details Handling Images back to top
|Alternative Name||CD80 (CD80 Antibody Abstract)|
Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Synonyms: Activation B7 1 antigen antibody|Activation B7-1 antigen antibody|Activation B71 antigen antibody|B lymphocyte activation antigen B7 antibody|B7 antibody|B7-1 antibody|B7-1 antigen antibody|BB1 antibody|CD 80 antibody|CD28 antigen ligand 1 antibody|CD28 antigen ligand 1 B7 1 antigen antibody|CD28 antigen ligand 1 B71 antigen antibody|CD28LG antibody|CD28LG1 antibody|CD80 antibody|CD80 antigen antibody|CD80 antigen precursor antibody|CD80 Molecule antibody|CD80_HUMAN antibody|Costimulatory factor CD80 antibody|CTLA 4 counter receptor B7.1 antibody|CTLA-4 counter-receptor B7.1 antibody|LAB 7 antibody|LAB7 antibody|T lymphocyte activation antigen CD80 antibody|T-lymphocyte activation antigen CD80 antibody
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process|
Application DetailsProduct Details anti-CD80 Antibody Target Details CD80 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, The detection limit for CD80 is approximately 1.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD80 Antibody Target Details CD80 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Sodium azide, Thimerosal (Merthiolate)|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-CD80 Antibody Target Details CD80 Application Details Handling back to top