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|Antigen||CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CREB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-CREB1 AntibodyTarget Details CREB1 Application Details Handling Images
|Sequence||Q-K-R-R-E-I-L-S-R-R-P-pS-Y-R-K-I-L-N-D-L-S-S-D-A-P correspond ing to aa 122-147|
|Specificity||This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.|
|Characteristics||Concentration: 0.064 mg/mL (by UV absorbance at 280 nm)|
|Immunogen||CREB phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
Target Details CREB1Product Details anti-CREB1 Antibody Application Details Handling Images back to top
|Alternative Name||CREB1 (CREB1 Antibody Abstract)|
|Background||The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1|
|Pathways||TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-CREB1 Antibody Target Details CREB1 Handling Images back to top
Western Blot: 1: 1,000. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.1 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|