anti-Rat (Rattus) PRKCE antibody for Immunofluorescence

Recommended PRKCE Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, epsilon (PRKCE) Antibodies
  • PKCE
  • nPKC-epsilon
  • 5830406C15Rik
  • PKC[e]
  • PKCepsilon
  • Pkce
  • R75156
  • fj59b07
  • prkce
  • wu:fj59b07
  • pkce
  • pkc-epsilon
  • PRKCH
  • protein kinase C epsilon
  • protein kinase C, epsilon
  • protein kinase C, epsilon a
  • PRKCE
  • Prkce
  • prkcea
  • prkce
Epitope
pSer729
26
21
15
11
10
10
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
185
104
83
21
17
5
2
2
2
2
1
1
Host
Rabbit
160
55
1
Clonality
Polyclonal
Conjugate
This PRKCE antibody is un-conjugated
10
10
6
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
175
79
77
39
37
27
26
16
15
9
8
4
3
2
1
Supplier
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Catalog No. ABIN6255486
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN6264427 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2755594 IC IF WB Rabbit C-Term, pSer729 Log in to see Polyclonal 0

General

Antigen Protein Kinase C, epsilon (PRKCE) Antibodies
Epitope pSer729
(26), (21), (15), (11), (10), (10), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(185), (104), (83), (21), (17), (5), (2), (2), (2), (2), (1), (1)
Host Rabbit
(160), (55), (1)
Clonality
Polyclonal
Conjugate This PRKCE antibody is un-conjugated
(10), (10), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(175), (79), (77), (39), (37), (27), (26), (16), (15), (9), (8), (4), (3), (2), (1)
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Product Details anti-PRKCE Antibody

Target Details PRKCE Application Details Handling Images
Specificity PKC epsilon (phospho S729) Antibody detects endogenous levels of PKC epsilon only when phosphorylated at S729
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human PKC epsilon (phospho S729)
Isotype IgG
Plasmids, Primers & others

Target Details PRKCE

Product Details anti-PRKCE Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKC epsilon (PRKCE Antibody Abstract)
Background

Description: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Gene: PRKCE

Molecular Weight 84 kDa
Gene ID 5581
UniProt Q02156
Pathways TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-PRKCE Antibody Target Details PRKCE Handling Images back to top
Application Notes WB 1:1000-3000 IHC 1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-PRKCE Antibody Target Details PRKCE Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-PRKCE Antibody Target Details PRKCE Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase C, epsilon (PRKCE) (pSer729) antibody (ABIN6255486) Western blot analysis of PKC epsilon (phospho S729) using HUVEC whole cell lysates
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Protein Kinase C, epsilon (PRKCE) (pSer729) antibody (ABIN6255486) ABIN6277780 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized...
Immunohistochemistry (IHC) image for anti-Protein Kinase C, epsilon (PRKCE) (pSer729) antibody (ABIN6255486) ABIN6277780 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde...