anti-Human ZAP70 antibody for Simple Western

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • SRK
  • STCD
  • STD
  • TZK
  • ZAP-70
  • 70kDa
  • AI327364
  • Srk
  • mrtle
  • mur
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • zgc:110383
  • zeta chain of T-cell receptor associated protein kinase 70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T cell receptor associated protein kinase 70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • tyrosine-protein kinase ZAP-70
  • ZAP70
  • Smp_149470
  • Zap70
  • zap70.L
  • zap70
  • LOC707555
  • LOC100342021
  • LOC100390922
  • LOC100542877
Epitope
AA 1-254
100
52
51
34
31
25
24
14
12
12
11
11
10
7
7
7
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
641
225
147
14
11
10
8
7
6
6
4
3
1
Host
Mouse
339
284
17
3
2
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is un-conjugated
33
27
25
21
15
14
7
7
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes)
338
216
206
204
140
136
65
52
50
17
8
7
4
4
3
2
2
2
1
Supplier
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Catalog No. ABIN4366573
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4366584 CyTOF FACS IF IHC (p) SimWes Mouse IgG2a kappa AA 1-254 Log in to see 2F3-2 0

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope AA 1-254
(100), (52), (51), (34), (31), (25), (24), (14), (12), (12), (11), (11), (10), (7), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(641), (225), (147), (14), (11), (10), (8), (7), (6), (6), (4), (3), (1)
Host Mouse
(339), (284), (17), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(33), (27), (25), (21), (15), (14), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes)
(338), (216), (206), (204), (140), (136), (65), (52), (50), (17), (8), (7), (4), (4), (3), (2), (2), (2), (1)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Specificity ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Purification Protein A or G purified
Immunogen Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
Clone 2F3-2
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background Gene Symbol: ZAP70
Molecular Weight Theoretical MW: 70 kDa
Gene ID 7535
UniProt P43403
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes Simple Western 10 μg/mL, Flow Cytometry 0.5 - 1 μg/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 μg/mL, Immunofluorescence 0.5 - 1.0 μg/mLHu-chromosome location: 2q12 Immunohistochemistry-Paraffin 0.5 - 1 μg/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Buffer 1.0 mM PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.